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TGFB2 elisa kit :: Human transforming growth factor, beta 2 ELISA Kit

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Catalog # MBS700392 (SPECIAL PROMOTIONAL PRICING for a limited time)
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $420 +1 FREE 8GB USB
  96-Strip-Wells  /  $600 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,495 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,715 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

transforming growth factor, beta 2 (TGFB2), ELISA Kit

Popular Item
Also Known As

Human transforming growth factors beta2, TGFbeta2 ELISA Kit

Product Synonym Names
Human transforming growth factors beta2; TGFbeta2 ELISA Kit; MGC116892; TGF-beta2; ; transforming growth factor; beta 2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS700392 Testing Data
Sequence Length
442
OMIM
gene 614816
3D Structure
ModBase 3D Structure for P61812
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human TGFB2. No significant cross-reactivity or interference between Human TGFB2 and analogues was observed.
Samples
Serum, plasma and cell culture supernates
Detection Range
15.6pg/ml-1000 pg/ml
Sensitivity
3.9 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TGFB2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTGFB2purchase
MBS700392 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the transforming growth factor, beta 2 (TGFB2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TGFB2. The ELISA analytical biochemical technique of the MBS700392 kit is based on TGFB2 antibody-TGFB2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TGFB2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TGFB2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TGFB2 elisa kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with anantibody specific to TGF-beta2. Standards or samples are then added tothe appropriate microtiter plate wells with a biotin-conjugatedantibody preparation specific for TGF-beta2 and Avidin conjugated toHorseradish Peroxidase (HRP) is added to each microplate well andincubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substratesolution is added to each well. Only those wells that contain TGF-beta2,biotin-conjugated antibody and enzyme-conjugated Avidin will exhibita change in color. The enzyme-substrate reaction is terminated bythe addition of a sulphuric acid solution and the color change ismeasured spectrophotometrically at a wavelength of 450 nm +/- 2 nm.The concentration of TGF-beta2 in the samples is then determined bycomparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of TGFB2 elisa kit
TGFB2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS700392. Click to request current manual
NCBI/Uniprot data below describe general gene information for TGFB2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
47,748 Da
NCBI Official Full Name
transforming growth factor beta-2 isoform 1
NCBI Official Synonym Full Names
transforming growth factor, beta 2
NCBI Official Symbol
TGFB2  [Similar Products]
NCBI Official Synonym Symbols
LDS4; TGF-beta2
  [Similar Products]
NCBI Protein Information
transforming growth factor beta-2; G-TSF; cetermin; polyergin; BSC-1 cell growth inhibitor; prepro-transforming growth factor beta-2; glioblastoma-derived T-cell suppressor factor
UniProt Protein Name
Transforming growth factor beta-2
UniProt Synonym Protein Names
BSC-1 cell growth inhibitor; Cetermin; Glioblastoma-derived T-cell suppressor factor; G-TSF; PolyerginCleaved into the following chain:Latency-associated peptide; LAP
UniProt Gene Name
TGFB2  [Similar Products]
UniProt Synonym Gene Names
TGF-beta-2; G-TSF; LAP  [Similar Products]
UniProt Entry Name
TGFB2_HUMAN
NCBI Summary for TGFB2
This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types by transducing their signal through combinations of transmembrane type I and type II receptors (TGFBR1 and TGFBR2) and their downstream effectors, the SMAD proteins. Disruption of the TGFB/SMAD pathway has been implicated in a variety of human cancers. The encoded protein is secreted and has suppressive effects of interleukin-2 dependent T-cell growth. Translocation t(1;7)(q41;p21) between this gene and HDAC9 is associated with Peters' anomaly, a congenital defect of the anterior chamber of the eye. The knockout mice lacking this gene show perinatal mortality and a wide range of developmental, including cardiac, defects. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2010]
UniProt Comments for TGFB2
Function: TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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