• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

TGL elisa kit :: Guinea Pig Triglyceride Lipase ELISA Kit

Scan QR to view Datasheet
Catalog # MBS737472
Unit / Price
Scan QR to view Datasheet
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Triglyceride Lipase (TGL), ELISA Kit

Full Product Name

Guinea pig Triglyceride Lipase ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
614025
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
5.0-100nmoL/L
Sensitivity
1.0nmoL/L
Intended Uses
This TGL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig TGL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TGL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for TGLpurchase
MBS737472 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Triglyceride Lipase (TGL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TGL. The ELISA analytical biochemical technique of the MBS737472 kit is based on TGL antibody-TGL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TGL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TGL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TGL elisa kit
Principle of the Assay: TGL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TGL antibody and an TGL-HRP conjugate. The assay sample and buffer are incubated together with TGL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TGL concentration since TGL from samples and TGL-HRP conjugate compete for the anti-TGL antibody binding site. Since the number of sites is limited, as more sites are occupied by TGL from the sample, fewer sites are left to bind TGL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TGL concentration in each sample is interpolated from this standard curve.

Typical Testing Data/Standard Curve (for reference only) of TGL elisa kit
TGL elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS737472. Click to request current manual
NCBI/Uniprot data below describe general gene information for TGL. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
55,914 Da
NCBI Official Full Name
triglyceride lipase
NCBI Official Synonym Full Names
lipase, hepatic
NCBI Official Symbol
NCBI Official Synonym Symbols
HL; HTGL; LIPH; HDLCQ12
  [Similar Products]
NCBI Protein Information
hepatic triacylglycerol lipase; hepatic lipase; lipase member C
UniProt Protein Name
Hepatic triacylglycerol lipase
UniProt Synonym Protein Names
Lipase member C
UniProt Gene Name
UniProt Synonym Gene Names
HTGL; HL; Hepatic lipase  [Similar Products]
UniProt Entry Name
LIPC_HUMAN
NCBI Summary for TGL
LIPC encodes hepatic triglyceride lipase, which is expressed in liver. LIPC has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. [provided by RefSeq, Jul 2008]
UniProt Comments for TGL
LIPC: Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin. Defects in LIPC are the cause of hepatic lipase deficiency (HL deficiency). A disorder characterized by elevated levels of beta-migrating very low density lipoproteins, and abnormally triglyceride-rich low and high density lipoproteins. Belongs to the AB hydrolase superfamily. Lipase family.

Protein type: Secreted, signal peptide; Secreted; Lipid Metabolism - glycerolipid; Phospholipase; EC 3.1.1.3

Chromosomal Location of Human Ortholog: 15q21-q23

Cellular Component: extracellular space

Molecular Function: heparin binding; triacylglycerol lipase activity; low-density lipoprotein binding; apolipoprotein binding; phospholipase activity

Biological Process: cholesterol metabolic process; cholesterol homeostasis; reverse cholesterol transport; triacylglycerol catabolic process; fatty acid biosynthetic process

Disease: High Density Lipoprotein Cholesterol Level Quantitative Trait Locus 12; Hepatic Lipase Deficiency; Diabetes Mellitus, Noninsulin-dependent
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000