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TGM2 elisa kit :: Rabbit TGM2 ELISA Kit

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Catalog # MBS2506706
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Typical Testing Data/Standard Curve (for reference only)
Product Name

TGM2, ELISA Kit

Popular Item
Full Product Name

Rabbit TGM2 (Transglutaminase 2, Tissue) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2506706 COA
OMIM
190196
Species Reactivity
Specificity
This kit recognizes natural and some recombinant Rabbit TGM2. No significant crossreactivity or interference between Rabbit TGM2 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
1.563-100ng/mL
Sensitivity
Min: 0.938ng/mL; Max: 100ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TGM2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for TGM2purchase
MBS2506706 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TGM2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TGM2. The ELISA analytical biochemical technique of the MBS2506706 kit is based on TGM2 antibody-TGM2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TGM2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TGM2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TGM2 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rabbit TGM2 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA method. The micro ELISA plate provided in this kit has been pre-coated with antibodies specific to Rabbit TGM2. Standards or samples added to appropriate micro ELISA plate wells will bind to the antibodies. Biotinylated detection antibodies specific to Rabbit TGM2 and Avidin-Horseradish Peroxidase (HRP) conjugate can then be added successively to each micro plate well. After incubation, free components are washed away. When the substrate solution is added to each well, only those wells that contain Rabbit TGM2, biotinylated detection antibody and Avidin-HRP complex will appear blue in color. The enzyme-substrate reaction will be terminated and appears yellow color by adding sulphuric acid solution. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit TGM2. You can calculate the concentration of Rabbit TGM2 in samples by comparing the OD of the samples with the standard curve.

Typical Testing Data/Standard Curve (for reference only) of TGM2 elisa kit
TGM2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2506706. Click to request current manual
NCBI/Uniprot data below describe general gene information for TGM2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
38,671 Da
NCBI Official Full Name
TGM2 protein, partial
NCBI Official Synonym Full Names
transglutaminase 2
NCBI Official Symbol
NCBI Official Synonym Symbols
TG2; TGC; GNAH; HEL-S-45; G-ALPHA-h
  [Similar Products]
NCBI Protein Information
protein-glutamine gamma-glutamyltransferase 2; C polypeptide, protein-glutamine-gamma-glutamyltransferase; TG(C); TGase C; TGase H; TGase-2; TGase-H; epididymis secretory protein Li 45; tissue transglutaminase; transglutaminase C; transglutaminase H; transglutaminase-2
UniProt Protein Name
Protein-glutamine gamma-glutamyltransferase 2
UniProt Synonym Protein Names
Tissue transglutaminase; Transglutaminase C; TG(C); TGC; TGase C; Transglutaminase H; TGase H; Transglutaminase-2; TGase-2
UniProt Gene Name
UniProt Synonym Gene Names
TG(C); TGC; TGase C; TGase H; TGase-2  [Similar Products]
UniProt Entry Name
TGM2_HUMAN
NCBI Summary for TGM2
Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for TGM2
TGM2: an enzyme of the transglutaminase family that catalyzes the crosslinking of proteins and the conjugation of polyamines to proteins. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. . Is the autoantigen in coeliac disease and plays a role in apoptosis, cellular differentiation and matrix stabilisation. Three alternatively spliced isoforms have been described.

Protein type: EC 2.3.2.13; Transferase

Chromosomal Location of Human Ortholog: 20q12

Cellular Component: focal adhesion; mitochondrion; plasma membrane; cytosol

Molecular Function: protein domain specific binding; protein binding; GTP binding; protein-glutamine gamma-glutamyltransferase activity; metal ion binding

Biological Process: elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of cell adhesion; positive regulation of apoptosis; isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of smooth muscle cell proliferation; blood vessel remodeling; apoptotic cell clearance; protein homooligomerization; elevation of mitochondrial calcium ion concentration; positive regulation of inflammatory response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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