• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

TH elisa kit :: Mouse Tyrosine Hydroxylase ELISA Kit

Scan QR to view Datasheet
Catalog # MBS738113
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Tyrosine Hydroxylase (TH), ELISA Kit

Popular Item
Also Known As

Mouse Tyrosine Hydroxylase ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 605407
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of TH. No significant cross-reactivity or interference between TH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TH and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTHpurchase
MBS738113 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Tyrosine Hydroxylase (TH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TH. The ELISA analytical biochemical technique of the MBS738113 kit is based on TH antibody-TH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TH elisa kit
Intended Uses: This TH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse TH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: TH ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-TH antibody and an TH-HRP conjugate. The assay sample and buffer are incubated together with TH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TH concentration since TH from samples and TH-HRP conjugate compete for the anti-TH antibody binding site. Since the number of sites is limited, as more sites are occupied by TH from the sample, fewer sites are left to bind TH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TH concentration in each sample is interpolated from this standard curve.
Product Categories/Family for TH elisa kit

Typical Testing Data/Standard Curve (for reference only) of TH elisa kit
TH elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS738113. Click to request current manual
NCBI/Uniprot data below describe general gene information for TH. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
44,898 Da
NCBI Official Full Name
tyrosine hydroxylase, partial
NCBI Official Synonym Full Names
tyrosine hydroxylase
NCBI Official Symbol
NCBI Official Synonym Symbols
TYH; DYT14; DYT5b
  [Similar Products]
NCBI Protein Information
tyrosine 3-monooxygenase; dystonia 14; tyrosine 3-hydroxylase
UniProt Protein Name
Tyrosine 3-monooxygenase
UniProt Synonym Protein Names
Tyrosine 3-hydroxylase; TH
UniProt Gene Name
UniProt Synonym Gene Names
TYH; TH  [Similar Products]
UniProt Entry Name
TY3H_HUMAN
NCBI Summary for TH
The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for TH
TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.

Protein type: Amino Acid Metabolism - tyrosine; Oxidoreductase; Vesicle; EC 1.14.16.2; Mitochondrial; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: synaptic vesicle; internal side of plasma membrane; neuron projection; smooth endoplasmic reticulum; mitochondrion; dendrite; melanosome membrane; cytoplasm; terminal button; perikaryon; cytoplasmic vesicle; cytosol; nucleus

Molecular Function: amino acid binding; protein domain specific binding; protein binding; enzyme binding; ferric iron binding; ferrous iron binding; dopamine binding; oxygen binding; tyrosine 3-monooxygenase activity

Biological Process: heart morphogenesis; response to peptide hormone stimulus; synaptic transmission, dopaminergic; heart development; response to lipopolysaccharide; locomotory behavior; dopamine biosynthetic process; fatty acid metabolic process; response to salt stress; phytoalexin metabolic process; response to estradiol stimulus; regulation of heart contraction; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; sensory perception of sound; catecholamine biosynthetic process; visual perception; response to electrical stimulus; epinephrine biosynthetic process; glycoside metabolic process; response to corticosterone stimulus; neurotransmitter biosynthetic process; response to pyrethroid; response to nutrient levels; response to light stimulus; anatomical structure morphogenesis; terpene metabolic process; sphingolipid metabolic process; eating behavior; phthalate metabolic process; response to amphetamine; mating behavior; multicellular organismal aging; isoquinoline alkaloid metabolic process; learning; social behavior; response to herbicide; response to ether; synaptic vesicle amine transport; memory; organ morphogenesis; eye photoreceptor cell development; response to ethanol; circadian sleep/wake cycle; pigmentation; response to zinc ion; response to hypoxia; embryonic camera-type eye morphogenesis; cerebral cortex development; response to activity; response to water deprivation

Disease: Segawa Syndrome, Autosomal Recessive
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000