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THBS1 elisa kit :: Mouse thrombospondin 1 ELISA Kit

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Catalog # MBS704061
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $575 +1 FREE 8GB USB
  96-Strip-Wells  /  $835 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,040 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,755 +7 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

thrombospondin 1 (THBS1), ELISA Kit

Popular Item
Full Product Name

Mouse thrombospondin 1, TSP-1 ELISA Kit

Product Synonym Names
Mouse thrombospondin 1; TSP-1 ELISA Kit; THBS; THBS-1; TSP; TSP-1; TSP1; thrombospondin-1p180; thrombospondin 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS704061 Testing Data
3D Structure
ModBase 3D Structure for P35441
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse TSP-1. No significant cross-reactivity or interference between mouse TSP-1 and analogues was observed.
Samples
Serum, plasma, cell culture supernates, tissue homogenates.
Assay Type
Sandwich (Quantitative)
Detection Range
7.8 pg/ml -500 pg/ml.
Sensitivity
1.95 pg/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of THBS1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTHBS1purchase
MBS704061 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the thrombospondin 1 (THBS1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing THBS1. The ELISA analytical biochemical technique of the MBS704061 kit is based on THBS1 antibody-THBS1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect THBS1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, THBS1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
THBS1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TSP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TSP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TSP-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of THBS1 elisa kit
THBS1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS704061. Click to request current manual
NCBI/Uniprot data below describe general gene information for THBS1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
129,647 Da
NCBI Official Full Name
Thrombospondin-1
NCBI Official Synonym Full Names
thrombospondin 1
NCBI Official Symbol
Thbs1  [Similar Products]
NCBI Official Synonym Symbols
TSP1; TSP-1; tbsp1; Thbs-1
  [Similar Products]
NCBI Protein Information
thrombospondin-1
UniProt Protein Name
Thrombospondin-1
Protein Family
UniProt Gene Name
Thbs1  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
TSP1_MOUSE
NCBI Summary for THBS1
The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
UniProt Comments for THBS1
Function: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Ligand for CD36 mediating antiangiogenic properties Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors.1 PublicationManual assertion based on experiment in:Ref.6
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withTHBS1 elisa kit
 Reference Product  PubMed Publications
 FLNA elisa kit  >12 publications with THBS1 and FLNA
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