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THRB sirna

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Catalog # MBS8229008
Unit / Price
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  15 nmol  /  $365 +1 FREE 8GB USB
  30 nmol  /  $515 +1 FREE 8GB USB
THRB sirna
Product Name

THRB, siRNA

Popular Item
Full Product Name

THRB siRNA (Human)

Product Synonym Names
ERBA2; NR1A2; THR1; Thyroid hormone receptor beta; Nuclear receptor subfamily 1 group A member 2; c-erbA-2; c-erbA-beta
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
145650
3D Structure
ModBase 3D Structure for P10828
Species Reactivity
Human
Specificity
THRB siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Source
Synthetic
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human THRB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of THRB sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
THRB sirna
siRNA to inhibit THRB expression using RNA interference
Applications Tested/Suitable for THRB sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for THRB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
54,449 Da
NCBI Official Full Name
thyroid hormone receptor beta
NCBI Official Synonym Full Names
thyroid hormone receptor, beta
NCBI Official Symbol
NCBI Official Synonym Symbols
GRTH; PRTH; THR1; ERBA2; NR1A2; THRB1; THRB2; C-ERBA-2; C-ERBA-BETA
  [Similar Products]
NCBI Protein Information
thyroid hormone receptor beta
UniProt Protein Name
Thyroid hormone receptor beta
UniProt Synonym Protein Names
Nuclear receptor subfamily 1 group A member 2; c-erbA-2; c-erbA-beta
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
ERBA2; NR1A2; THR1  [Similar Products]
UniProt Entry Name
THB_HUMAN
NCBI Summary for THRB
The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Mutations in this gene are known to be a cause of generalized thyroid hormone resistance (GTHR), a syndrome characterized by goiter and high levels of circulating thyroid hormone (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH). Several alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for THRB
TR-beta1: nuclear hormone receptor and transcription factor. High affinity receptor for triiodothyronine. Homolog of the viral oncogene erbB. Two splice variant isoforms have been described.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 3p24.2

Cellular Component: nucleoplasm; nuclear chromatin; nucleus

Molecular Function: protein binding; enzyme binding; DNA binding; zinc ion binding; sequence-specific DNA binding; chromatin DNA binding; steroid hormone receptor activity; thyroid hormone receptor activity; transcription factor activity; transcription corepressor activity

Biological Process: negative regulation of female receptivity; transcription initiation from RNA polymerase II promoter; organ morphogenesis; sensory perception of sound; female courtship behavior; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; gene expression; steroid hormone mediated signaling; negative regulation of transcription from RNA polymerase II promoter; regulation of heart contraction

Disease: Thyroid Hormone Resistance, Selective Pituitary; Thyroid Hormone Resistance, Generalized, Autosomal Dominant; Thyroid Hormone Resistance, Generalized, Autosomal Recessive
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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