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TIMP-1 elisa kit :: Goat Tissue Inhibitors Of Metalloproteinase 1 (TIMP-1) ELISA Kit

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Catalog # MBS266813
Unit / Price
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  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $475 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 Typical Testing Data/Standard Curve (for reference only)
Product Name

Tissue Inhibitors Of Metalloproteinase 1 (TIMP-1), ELISA Kit

Popular Item
Full Product Name

Goat Tissue Inhibitors Of Metalloproteinase 1 (TIMP-1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
305370
Species Reactivity
Specificity
No cross-reaction with other factors.
Samples
Serum, plasma or cell culture supernatant
Assay Type
Quantitative Sandwich
Detection Range
10 ng/ml-0.156 ng/ml
Sensitivity
0.05 ng/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TIMP-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for TIMP-1purchase
MBS266813 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Tissue Inhibitors Of Metalloproteinase 1 (TIMP-1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TIMP-1. The ELISA analytical biochemical technique of the MBS266813 kit is based on TIMP-1 antibody-TIMP-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TIMP-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TIMP-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TIMP-1 elisa kit
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Goat TIMP-1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve (for reference only) of TIMP-1 elisa kit
TIMP-1 elisa kit  Typical Testing Data/Standard Curve (for reference only) image
NCBI/Uniprot data below describe general gene information for TIMP-1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
23,171 Da
NCBI Official Full Name
tissue inhibitor of metalloproteinase 1, partial
NCBI Official Synonym Full Names
TIMP metallopeptidase inhibitor 1
NCBI Official Symbol
TIMP1  [Similar Products]
NCBI Official Synonym Symbols
EPA; EPO; HCI; CLGI; TIMP
  [Similar Products]
NCBI Protein Information
metalloproteinase inhibitor 1; TIMP-1; collagenase inhibitor; erythroid potentiating activity; erythroid-potentiating activity; fibroblast collagenase inhibitor; tissue inhibitor of metalloproteinases 1
UniProt Protein Name
Metalloproteinase inhibitor 1
UniProt Synonym Protein Names
Erythroid-potentiating activity; EPA; Fibroblast collagenase inhibitor; Collagenase inhibitor; Tissue inhibitor of metalloproteinases 1; TIMP-1
UniProt Gene Name
TIMP1  [Similar Products]
UniProt Synonym Gene Names
CLGI; TIMP; EPA; Collagenase inhibitor; TIMP-1  [Similar Products]
UniProt Entry Name
TIMP1_HUMAN
NCBI Summary for TIMP-1
This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008]
UniProt Comments for TIMP-1
TIMP1: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xp11.3-p11.23

Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region; basement membrane

Molecular Function: metalloendopeptidase inhibitor activity; protein binding; protease binding; growth factor activity; metal ion binding; cytokine activity

Biological Process: response to peptide hormone stimulus; platelet activation; extracellular matrix disassembly; extracellular matrix organization and biogenesis; negative regulation of metalloenzyme activity; platelet degranulation; response to cytokine stimulus; positive regulation of cell proliferation; response to hormone stimulus; blood coagulation; negative regulation of membrane protein ectodomain proteolysis; negative regulation of apoptosis; aging
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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