• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

TJP1 elisa kit :: Mouse tight junction protein 1 (zona occludens 1) ELISA Kit

Scan QR to view Datasheet
Catalog # MBS9324491
Unit / Price
Scan QR to view Datasheet
  48-Strip-Wells  /  $470 +1 FREE 8GB USB
  96-Strip-Wells  /  $680 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
TJP1 elisa kit
Product Name

tight junction protein 1 (zona occludens 1) (TJP1), ELISA Kit

Popular Item
Full Product Name

Mouse Tight junction protein ZO-1, TJP1 ELISA Kit

Product Synonym Names
Mouse Tight junction protein ZO-1 (TJP1) ELISA kit; DKFZp686M05161; MGC133289; ZO-1; tight junction protein 1; tight junction protein ZO-1; zona occludens 1; zonula occludens 1 protein; tight junction protein 1 (zona occludens 1)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS9324491 COA
3D Structure
ModBase 3D Structure for P39447
Species Reactivity
Specificity
No significant cross-reactivity or interference between this analyte and analogues is observed.
Samples
Undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
Assay Type
Sandwich
Detection Range
0.25 ng/ml - 8 ng/ml.
Sensitivity
0.1 ng/ml.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TJP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for TJP1purchase
MBS9324491 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tight junction protein 1 (zona occludens 1) (TJP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TJP1. The ELISA analytical biochemical technique of the MBS9324491 kit is based on TJP1 antibody-TJP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TJP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TJP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TJP1 elisa kit
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of TJP1 (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
Sample Manual Insert of MBS9324491. Click to request current manual
NCBI/Uniprot data below describe general gene information for TJP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
194,742 Da
NCBI Official Full Name
tight junction protein ZO-1 isoform 1
NCBI Official Synonym Full Names
tight junction protein 1
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
tight junction protein ZO-1; zona occludens protein 1; zonula occludens protein 1
UniProt Protein Name
Tight junction protein ZO-1
UniProt Synonym Protein Names
Tight junction protein 1; Zona occludens protein 1; Zonula occludens protein 1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
ZO1_MOUSE
UniProt Comments for TJP1
ZO1: a scaffolding protein of the MAGUK (membrane-associated guanylate kinase) family associated with tight junctions (TJ). May mediate dynamic changes in the composition of TJs. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting the protein kinase MRCKb to the leading edge of migrating cells. Interacts with BVES (via the C-terminus cytoplasmic tail). Interacts with HSPA4 and KIRREL1. Forms homodimers and heterodimers with ZO-2 or ZO-3. Interacts with occludin, CALM, claudins, CGN, CXADR, GJA12, GJD3 and UBN1. Interacts (via ZU5 domain) with MRCKb and MYZAP. Interacts (via PDZ domain) with Cx43. ZO-2 and ZO-3 shuttle between the TJ and the nucleus, where they may regulate gene expression. TJ, or zonula occludens, are the closely associated areas of two cells whose membranes are tightly joined together. They are composed of a branching network of strands, the efficiency of which increases exponentially with the number of strands. The strands associate with transmembrane proteins that bind to similar proteins on adjacent cells, and with submembranous proteins that are anchored to the actin component of the cytoskeleton. Thus, TJs join together the cytoskeletons of adjacent cells. They endow tissues with substantial form, shape and location, enforce cellular polarity, and form barriers to molecules and pathogens. Molecules forming the membranous part of TJs include occludin, claudins, tricellulin and junctional adhesion molecules. These molecules interact with the proteins ZO-1, ZO-2 and ZO-3. Tight junction proteins can be up- or downregulated in cancer and are involved in the epithelial-mesenchymal transitions (EMT) in tumors. Alternative splicing produces two isoforms. The long isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Cellular Component: apical junction complex; intercellular canaliculus; tight junction; cell surface; apicolateral plasma membrane; basolateral plasma membrane; intercellular junction; cell-cell adherens junction; adherens junction; membrane; apical part of cell; apical plasma membrane; cytoplasm; gap junction; plasma membrane; nucleus; cell junction

Molecular Function: calmodulin binding; protein C-terminus binding; protein domain specific binding; protein binding

Biological Process: sensory perception of sound; blastocyst formation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000