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TLR2 elisa kit :: Monkey Toll Like Receptor2 ELISA Kit

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Catalog # MBS743183
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Toll Like Receptor2 (TLR2), ELISA Kit

Popular Item
Full Product Name

Monkey Toll Like Receptor2 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
603028
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Detection Range
1.0-25ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TLR2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for TLR2purchase
MBS743183 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Toll Like Receptor2 (TLR2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TLR2. The ELISA analytical biochemical technique of the MBS743183 kit is based on TLR2 antibody-TLR2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TLR2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TLR2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TLR2 elisa kit
Intended Uses: This TLR-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey TLR-2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: TLR-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TLR-2 antibody and an TLR-2-HRP conjugate. The assay sample and buffer are incubated together with TLR-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TLR-2 concentration since TLR-2 from samples and TLR-2-HRP conjugate compete for the anti-TLR-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by TLR-2 from the sample, fewer sites are left to bind TLR-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TLR-2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for TLR2 elisa kit

Typical Testing Data/Standard Curve (for reference only) of TLR2 elisa kit
TLR2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS743183. Click to request current manual
NCBI/Uniprot data below describe general gene information for TLR2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
89,838 Da
NCBI Official Full Name
toll-like receptor 2
NCBI Official Synonym Full Names
toll-like receptor 2
NCBI Official Symbol
NCBI Official Synonym Symbols
TIL4; CD282
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NCBI Protein Information
toll-like receptor 2; toll/interleukin 1 receptor-like 4; toll/interleukin-1 receptor-like protein 4
UniProt Protein Name
Toll-like receptor 2
UniProt Synonym Protein Names
Toll/interleukin-1 receptor-like protein 4; CD_antigen: CD282
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
TLR2_HUMAN
NCBI Summary for TLR2
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. [provided by RefSeq, Jul 2008]
UniProt Comments for TLR2
TLR2: Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage- activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. Interacts with LY96, TLR1 and TLR6 (via extracellular domain). Binds MYD88 (via TIR domain). Interacts with TICAM1. Ligand binding induces the formation of a heterodimer with TLR1. Interacts with CNPY3. Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues. Belongs to the Toll-like receptor family.

Protein type: Motility/polarity/chemotaxis; Receptor, misc.; Membrane protein, integral; Apoptosis; Cell surface

Chromosomal Location of Human Ortholog: 4q32

Cellular Component: cell projection; cell surface; integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: peptidoglycan binding; protein binding; transmembrane receptor activity; protein heterodimerization activity; triacylated lipoprotein binding; lipopolysaccharide receptor activity; receptor activity; pattern recognition receptor activity; diacylated lipoprotein binding

Biological Process: positive regulation of nitric oxide biosynthetic process; apoptosis; positive regulation of interleukin-12 production; response to toxin; positive regulation of leukocyte migration; microglial cell activation; positive regulation of NF-kappaB import into nucleus; leukotriene metabolic process; detection of triacylated bacterial lipoprotein; response to molecule of fungal origin; positive regulation of interleukin-18 production; signal transduction; nitric oxide metabolic process; positive regulation of interleukin-10 production; activation of NF-kappaB transcription factor; response to insulin stimulus; positive regulation of interleukin-8 production; negative regulation of cell proliferation; lipopolysaccharide-mediated signaling pathway; positive regulation of interferon-beta production; positive regulation of oligodendrocyte differentiation; inflammatory response; positive regulation of Wnt receptor signaling pathway; toll-like receptor 4 signaling pathway; positive regulation of tumor necrosis factor biosynthetic process; negative regulation of interleukin-12 production; detection of diacylated bacterial lipoprotein; cell surface pattern recognition receptor signaling pathway; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of toll-like receptor signaling pathway; positive regulation of chemokine production; toll-like receptor 2 signaling pathway; induction by symbiont of defense-related host nitric oxide production; MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; negative regulation of interleukin-17 production; myelin formation in the central nervous system; response to hypoxia; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; immune response; I-kappaB phosphorylation; response to progesterone stimulus; chloramphenicol transport; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of cytokine secretion; positive regulation of inflammatory response

Disease: Leprosy, Susceptibility To, 3; Mycobacterium Tuberculosis, Susceptibility To
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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