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TLR4 recombinant protein :: Toll Like Receptor 4 (TLR4) Recombinant Protein

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Catalog # MBS2009564 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $130 +1 FREE 8GB USB
  0.05 mg  /  $225 +1 FREE 8GB USB
  0.1 mg  /  $340 +1 FREE 8GB USB
  0.2 mg  /  $415 +1 FREE 8GB USB
  0.5 mg  /  $800 +1 FREE 8GB USB
  1 mg  /  $1,180 +1 FREE 8GB USB
Product Name

Toll Like Receptor 4 (TLR4), Recombinant Protein

Popular Item
Full Product Name

Recombinant Toll Like Receptor 4 (TLR4)

Matching Pairs
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS2009564 Technical Datasheet
Chromosome Location
Chromosome: 4; NC_000070.6 (66827811..66842797). Location: 4 C1; 4 34.66 cM
3D Structure
ModBase 3D Structure for Q9QUK6
Host
E Coli
Purity/Purification
> 95%
Form/Format
10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
Concentration
Original Concentration: 200 ug/mL (lot specific)
Organism Species
Mus musculus (Mouse)
Source
Prokaryotic expression
Residues
Asn26~Glu269
Tags
N-terminal His Tag
Subcellular Location
Membrane
Traits
Freeze-dried powder
Predicted isoelectric point
5.6
Predicted Molecular Mass
33.3kDa
Accurate Molecular Mass
32kDa as determined by SDS-PAGE reducing conditions.
Usage
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of TLR4 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for TLR4 recombinant protein
Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Sequence Information of TLR4 recombinant protein
TLR4 recombinant protein Sequence Information image
NCBI/Uniprot data below describe general gene information for TLR4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
toll-like receptor 4
NCBI Official Synonym Full Names
toll-like receptor 4
NCBI Official Symbol
NCBI Official Synonym Symbols
Lps; Ly87; Ran/M1; Rasl2-8
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NCBI Protein Information
toll-like receptor 4; lipopolysaccharide response
UniProt Protein Name
Toll-like receptor 4
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
TLR4_MOUSE
NCBI Summary for TLR4
This gene belongs to the evolutionarily-conserved Toll-like receptor family, whose members are type-1 transmembrane proteins that are involved in innate immunity. Toll-like receptors are characterized by an extracellular leucine-rich repeat domain that functions in ligand recognition and an intracellular toll/interleukin-1 receptor-like domain that is crucial for signal transduction. The receptor encoded by this gene mediates the innate immune response to bacterial lipopolysaccharide, a major component of the outer membrane of Gram-negative bacteria, through synthesis of pro-inflammatory cytokines and chemokines. In addition, this protein can recognize other pathogens from Gram-negative and Gram-positive bacteria as well as viral components. Mice deficient in this gene display a number of immune response-related phenotypes including hyporesponsiveness to bacterial lipopolysaccharide and increased levels of respiratory syncytial virus compared to controls. [provided by RefSeq, Sep 2015]
UniProt Comments for TLR4
TLR4: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS- independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4. Binding to bacterial LPS leads to homodimerization. Interacts with LY96 via the extracellular domain. Interacts with MYD88 and TIRAP via their respective TIR domains. Interacts with NOX4. Interacts with CNPY3. Interacts with HSP90B1. The interaction with both CNPY3 and HSP90B1 is required for proper folding in the endoplasmic reticulum. Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. Belongs to the Toll-like receptor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Cellular Component: cell surface; membrane; perinuclear region of cytoplasm; integral to plasma membrane; cytoplasm; integral to membrane; plasma membrane; lipopolysaccharide receptor complex; lipid raft; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; lipopolysaccharide binding; lipopolysaccharide receptor activity; phosphoinositide 3-kinase binding; receptor activity

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of apoptosis; activation of MAPK activity; leukotriene metabolic process; response to lipopolysaccharide; positive regulation of NF-kappaB import into nucleus; positive regulation of interleukin-10 production; activation of NF-kappaB transcription factor; positive regulation of B cell proliferation; positive regulation of interferon-beta production; negative regulation of interleukin-6 production; toll-like receptor 4 signaling pathway; positive regulation of interferon-alpha production; positive regulation of DNA binding; positive regulation of I-kappaB kinase/NF-kappaB cascade; production of nitric oxide during acute inflammatory response; positive regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; macrophage activation; negative regulation of interleukin-23 production; positive regulation of peptidyl-tyrosine phosphorylation; response to ethanol; response to bacterium; detection of lipopolysaccharide; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; response to oxidative stress; I-kappaB phosphorylation; activation of innate immune response; positive regulation of nitric-oxide synthase biosynthetic process; regulation of cytokine secretion; positive regulation of interleukin-12 production; positive regulation of interleukin-6 biosynthetic process; microglial cell activation; positive regulation of JNK cascade; signal transduction; defense response to Gram-negative bacterium; mast cell activation; positive regulation of interleukin-1 biosynthetic process; positive regulation of stress-activated MAPK cascade; positive regulation of interleukin-8 production; positive regulation of lymphocyte proliferation; positive regulation of interleukin-13 biosynthetic process; positive regulation of MHC class II biosynthetic process; negative regulation of interferon-gamma production; lipopolysaccharide-mediated signaling pathway; interferon-gamma production; inflammatory response; innate immune response-activating signal transduction; B cell proliferation during immune response; positive regulation of tumor necrosis factor biosynthetic process; immune system process; activation of NF-kappaB-inducing kinase; negative regulation of tumor necrosis factor production; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interferon-gamma production; negative regulation of interleukin-17 production; positive regulation of interleukin-12 biosynthetic process; positive regulation of interferon-beta biosynthetic process; regulation of inflammatory response; toll-like receptor signaling pathway; innate immune response; immune response; regulation of sensory perception of pain; positive regulation of interleukin-1 production; positive regulation of inflammatory response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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