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TLR9 recombinant protein :: Toll Like Receptor 9 Recombinant Protein

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Catalog # MBS2031580 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $140 +1 FREE 8GB USB
  0.05 mg  /  $260 +1 FREE 8GB USB
  0.1 mg  /  $400 +1 FREE 8GB USB
  0.2 mg  /  $495 +1 FREE 8GB USB
  0.5 mg  /  $955 +1 FREE 8GB USB
  1 mg  /  $1,415 +1 FREE 8GB USB
Product Name

Toll Like Receptor 9 (TLR9), Recombinant Protein

Popular Item
Also Known As

Recombinant Toll Like Receptor 9 (TLR9)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS2031580 Technical Datasheet
Host
E. coli
Purity/Purification
> 97%
Concentration
Original Concentration: 200 ug/mL (lot specific)
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression
Residues
Thr28~Ser362
Tags
N-terminal His Tag
Subcellular Location
Lysosome, Cytoplasm, Endoplasmic reticulum lumen, Endosome
Traits
Freeze-dried powder
Buffer
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% SKL, 5% Trehalose and Proclin300.
Predicted isoelectric point
9.1
Predicted Molecular Mass
41.1kDa
Accurate Molecular Mass
41kDa as determined by SDS-PAGE reducing conditions.
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition
Other Notes
Small volumes of TLR9 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for TLR9 recombinant protein
Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Sequence Information of TLR9 recombinant protein
TLR9 recombinant protein Sequence Information image
Testing Data of TLR9 recombinant protein
TLR9 recombinant protein Testing Data image
NCBI/Uniprot data below describe general gene information for TLR9. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
toll-like receptor 9
NCBI Official Synonym Full Names
toll-like receptor 9
NCBI Official Symbol
NCBI Protein Information
toll-like receptor 9
UniProt Protein Name
Toll-like receptor 9
UniProt Synonym Protein Names
CD_antigen: CD289
Protein Family
UniProt Gene Name
UniProt Entry Name
TLR9_MOUSE
UniProt Comments for TLR9
TLR9: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Controls lymphocyte response to Helicobacter infection. Interacts with MYD88 via their respective TIR domains. Interacts (via transmembrane domain) with UNC93B1. Interacts with CD300LH; the interaction may promote full activation of TLR9-triggered innate responses. Interacts with BTK. Interacts with CNPY3 and HSP90B1; this interaction is required for proper folding in the endoplasmic reticulum. Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre- dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver. Belongs to the Toll-like receptor family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Cellular Component: membrane; basolateral plasma membrane; endoplasmic reticulum; lysosome; cytoplasm; apical plasma membrane; plasma membrane; integral to membrane; cytoplasmic vesicle; endosome

Molecular Function: protein binding; transmembrane receptor activity; siRNA binding; interleukin-1 receptor binding; unmethylated CpG binding

Biological Process: positive regulation of JNK activity; regulation of cytokine secretion; positive regulation of interleukin-12 production; maintenance of gastrointestinal epithelium; microglial cell activation; positive regulation of interferon-alpha biosynthetic process; regulation of B cell activation; positive regulation of NF-kappaB import into nucleus; signal transduction; positive regulation of interleukin-18 production; defense response to Gram-negative bacterium; positive regulation of interleukin-10 production; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; response to molecule of bacterial origin; positive regulation of interferon-beta production; negative regulation of interleukin-8 production; negative regulation of interleukin-6 production; inflammatory response; defense response to virus; positive regulation of autophagy; regulation of protein amino acid phosphorylation; positive regulation of I-kappaB kinase/NF-kappaB cascade; immune system process; response to virus; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of toll-like receptor signaling pathway; positive regulation of chemokine production; MyD88-dependent toll-like receptor signaling pathway; inhibition of NF-kappaB transcription factor; regulation of inflammatory response; positive regulation of interferon-beta biosynthetic process; negative regulation of toll-like receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of inflammatory response; positive regulation of interferon-gamma biosynthetic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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