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TMED1 elisa kit :: Mouse TMED1 ELISA Kit

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Catalog # MBS2508644
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

TMED1, ELISA Kit

Popular Item
Full Product Name

Mouse TMED1 (Transmembrane Emp24 Domain Containing Protein 1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
605395
3D Structure
ModBase 3D Structure for Q13445
Species Reactivity
Specificity
This kit recognizes natural and recombinant Mouse TMED1. No significant cross-reactivity or interference between Mouse TMED1 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
Min: 0.094ng/mL; Max: 10ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TMED1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTMED1purchase
MBS2508644 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TMED1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TMED1. The ELISA analytical biochemical technique of the MBS2508644 kit is based on TMED1 antibody-TMED1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TMED1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TMED1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TMED1 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse TMED1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TMED1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TMED1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TMED1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TMED1. You can calculate the concentration of TMED1 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of TMED1 elisa kit
TMED1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2508644. Click to request current manual
NCBI/Uniprot data below describe general gene information for TMED1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
25,206 Da
NCBI Official Full Name
transmembrane emp24 domain-containing protein 1
NCBI Official Synonym Full Names
transmembrane emp24 protein transport domain containing 1
NCBI Official Symbol
TMED1  [Similar Products]
NCBI Official Synonym Symbols
Tp24; Il1rl1l; IL1RL1LG
  [Similar Products]
NCBI Protein Information
transmembrane emp24 domain-containing protein 1; IL1RL1-binding protein; interleukin 1 receptor-like 1 ligand; p24 family protein gamma-1; putative T1/ST2 receptor-binding protein
UniProt Protein Name
Transmembrane emp24 domain-containing protein 1
UniProt Synonym Protein Names
Interleukin-1 receptor-like 1 ligand; Putative T1/ST2 receptor-binding protein; p24 family protein gamma-1; Tp24; p24gamma1
UniProt Gene Name
TMED1  [Similar Products]
UniProt Synonym Gene Names
IL1RL1L; IL1RL1LG; Tp24; p24gamma1  [Similar Products]
UniProt Entry Name
TMED1_HUMAN
NCBI Summary for TMED1
This gene belongs to the TMED (transmembrane emp24 domain-containing) protein family, which is involved in the vesicular trafficking of proteins. The protein encoded by this gene was identified by its interaction with interleukin 1 receptor-like 1 (IL1RL1) and may play a role in innate immunity. This protein lacks any similarity to other interleukin 1 ligands. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
UniProt Comments for TMED1
TMED1: Potential role in vesicular protein trafficking, mainly in the early secretory pathway. May act as a cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and may be involved in vesicle coat formation at the cytoplasmic side. Belongs to the EMP24/GP25L family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; endoplasmic reticulum; plasma membrane; integral to membrane; ER-Golgi intermediate compartment

Molecular Function: protein binding; receptor binding

Biological Process: protein transport; cell-cell signaling; signal transduction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withTMED1 elisa kit
 Reference Product  PubMed Publications
 IL1RL1 elisa kit  >1 publications with TMED1 and IL1RL1
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