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TMEM195 elisa kit :: Mouse Alkylglycerol monooxygenase (TMEM195) ELISA Kit

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Catalog # MBS7244380
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Alkylglycerol monooxygenase (TMEM195), ELISA Kit

Popular Item
Full Product Name

Mouse Alkylglycerol monooxygenase (TMEM195) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
613738
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of TMEM195. No significant cross-reactivity or interference between TMEM195 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TMEM195 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TMEM195 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTMEM195purchase
MBS7244380 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alkylglycerol monooxygenase (TMEM195) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TMEM195. The ELISA analytical biochemical technique of the MBS7244380 kit is based on TMEM195 antibody-TMEM195 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TMEM195 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TMEM195. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TMEM195 elisa kit
Intended Uses: This TMEM195 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse TMEM195. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: TMEM195 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-TMEM195 antibody and an TMEM195-HRP conjugate. The assay sample and buffer are incubated together with TMEM195-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TMEM195 concentration since TMEM195 from samples and TMEM195-HRP conjugate compete for the anti-TMEM195 antibody binding site. Since the number of sites is limited, as more sites are occupied by TMEM195 from the sample, fewer sites are left to bind TMEM195-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TMEM195 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for TMEM195 elisa kit

Typical Testing Data/Standard Curve (for reference only) of TMEM195 elisa kit
TMEM195 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7244380. Click to request current manual
NCBI/Uniprot data below describe general gene information for TMEM195. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
51,500 Da
NCBI Official Full Name
alkylglycerol monooxygenase
NCBI Official Synonym Full Names
alkylglycerol monooxygenase
NCBI Official Symbol
NCBI Official Synonym Symbols
TMEM195
  [Similar Products]
NCBI Protein Information
alkylglycerol monooxygenase; transmembrane protein 195; glyceryl-ether monooxygenase
UniProt Protein Name
Alkylglycerol monooxygenase
UniProt Synonym Protein Names
Transmembrane protein 195
UniProt Gene Name
UniProt Synonym Gene Names
TMEM195  [Similar Products]
UniProt Entry Name
ALKMO_HUMAN
NCBI Summary for TMEM195
The protein encoded by this gene is a tetrahydrobiopterin- and iron-dependent enzyme that cleaves the ether bond of alkylglycerols. Sequence comparisons distinguish this protein as forming a third, distinct class of tetrahydrobiopterin-dependent enzymes. Variations in this gene have been associated with decreased glucose-stimulated insulin response, type 2 diabetes, and susceptibility to intracranial aneurysms. [provided by RefSeq, Aug 2012]
UniProt Comments for TMEM195
AGMO: Glyceryl-ether monooxygenase that cleaves the O-alkyl bond of ether lipids. Ether lipids are essential components of brain membranes. Belongs to the sterol desaturase family. TMEM195 subfamily.

Protein type: Oxidoreductase; Endoplasmic reticulum; Membrane protein, multi-pass; Membrane protein, integral; EC 1.14.16.5

Chromosomal Location of Human Ortholog: 7p21.2

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function: iron ion binding; glyceryl-ether monooxygenase activity

Biological Process: ether lipid metabolic process; membrane lipid metabolic process; fatty acid biosynthetic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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