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TNFAIP3 blocking peptide :: A20 Blocking Peptide

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Catalog # MBS823308
Unit / Price
  1 mg  /  $160 +1 FREE 8GB USB
  5 mg  /  $305 +1 FREE 8GB USB
TNFAIP3 blocking peptide
Product Name

A20 (TNFAIP3), Blocking Peptide

Popular Item
Also Known As

A20 Blocking Peptide

Product Synonym Names
OTUD7C; Tumor necrosis factor alpha-induced protein 3; TNF alpha-induced protein 3; OTU domain-containing protein 7C; Putative DNA-binding protein A20; Zinc finger protein A20
Antibody/Peptide Pairs
A20 peptide (MBS823308) is used for blocking the activity of A20 antibody (MBS820465)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
790
OMIM
191163
3D Structure
ModBase 3D Structure for P21580
Host
Synthetic
Species Reactivity
Human, Mouse, Rat
Purity/Purification
>85%
Form/Format
Lyophilized powder
Quality Control
The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Other Notes
Small volumes of TNFAIP3 blocking peptide vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TNFAIP3 blocking peptide
The peptide is used to block Anti-A20 Antibody reactivity.
Applications Tested/Suitable for TNFAIP3 blocking peptide
Blocking (BL)
Application Notes for TNFAIP3 blocking peptide
Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4 degree C for overnight or at room temperature for 2 hours.
NCBI/Uniprot data below describe general gene information for TNFAIP3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
89,614 Da
NCBI Official Full Name
tumor necrosis factor alpha-induced protein 3
NCBI Official Synonym Full Names
tumor necrosis factor, alpha-induced protein 3
NCBI Official Symbol
TNFAIP3  [Similar Products]
NCBI Official Synonym Symbols
A20; OTUD7C; TNFA1P2
  [Similar Products]
NCBI Protein Information
tumor necrosis factor alpha-induced protein 3; zinc finger protein A20; TNF alpha-induced protein 3; OTU domain-containing protein 7C; putative DNA-binding protein A20; tumor necrosis factor inducible protein A20
UniProt Protein Name
Tumor necrosis factor alpha-induced protein 3
UniProt Synonym Protein Names
OTU domain-containing protein 7C; Putative DNA-binding protein A20; Zinc finger protein A20Cleaved into the following 2 chains:A20p50; A20p37
UniProt Gene Name
TNFAIP3  [Similar Products]
UniProt Synonym Gene Names
OTUD7C; TNF alpha-induced protein 3  [Similar Products]
UniProt Entry Name
TNAP3_HUMAN
NCBI Summary for TNFAIP3
This gene was identified as a gene whose expression is rapidly induced by the tumor necrosis factor (TNF). The protein encoded by this gene is a zinc finger protein and ubiqitin-editing enzyme, and has been shown to inhibit NF-kappa B activation as well as TNF-mediated apoptosis. The encoded protein, which has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2012]
UniProt Comments for TNFAIP3
TNFAIP3: Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Homodimer. Interacts with TNIP1, TAX1BP1 and TRAF2. Interacts with RNF11, ITCH and TAX1BP1 only after TNF stimulation; these interaction are transient and they are lost after 1 hour of stimulation with TNF. Interacts with YWHAZ and YWHAH. Interacts with IKBKG; the interaction is induced by TNF stimulation and by polyubiquitin. Interacts with RIPK1. Interacts with UBE2N; the interaction requires TAX1BP1. Interacts with TRAF6; the interaction is inhibited by HTLV-1 protein Tax. By TNF. Belongs to the peptidase C64 family.

Protein type: Apoptosis; Ubiquitin conjugating system; Protease; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 6q23

Cellular Component: centrosome; lysosome; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; ubiquitin binding; protein self-association; DNA binding; protease binding; zinc ion binding; ubiquitin-specific protease activity; ubiquitin-protein ligase activity; kinase binding; ligase activity

Biological Process: negative regulation of toll-like receptor 5 signaling pathway; negative regulation of smooth muscle cell proliferation; apoptosis; proteolysis; negative regulation of toll-like receptor 3 signaling pathway; negative regulation of interleukin-2 production; negative regulation of innate immune response; response to molecule of bacterial origin; negative regulation of interleukin-6 production; negative regulation of bone resorption; inflammatory response; negative regulation of chronic inflammatory response; protein deubiquitination; negative regulation of toll-like receptor 2 signaling pathway; B-1 B cell homeostasis; negative regulation of cyclin-dependent protein kinase activity; negative regulation of B cell activation; negative regulation of tumor necrosis factor production; negative regulation of interleukin-1 beta production; negative regulation of I-kappaB kinase/NF-kappaB cascade; protein oligomerization; regulation of germinal center formation; response to muramyl dipeptide; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; negative regulation of toll-like receptor 4 signaling pathway; positive regulation of protein catabolic process; innate immune response; negative regulation of protein ubiquitination; regulation of defense response to virus by host; negative regulation of interferon type I production
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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