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TNFalpha elisa kit :: Human TNFalpha ELISA Kit

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Catalog # MBS355357
Unit / Price
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  96 Tests  /  $385 +1 FREE 8GB USB
 Typical Testing Data/Standard Curve (for reference only)
Product Name

TNFalpha, ELISA Kit

Popular Item
Full Product Name

TNFalpha Human ELISA Kit

Product Synonym Names
Human TNFalpha
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Species Reactivity
Samples
Human serum, body fluids, tissue lysates or cell culture supernatants
Assay Type
Quantitative Sandwich
Detection Range
15.6 pg/ml-1000 pg/ml (Body fluids, tissue lysates or cell culture supernates) 7.8 pg/ml-500 pg/ml (Human serum)
Sensitivity
< 1 pg/ml
Preparation and Storage
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TNFalpha elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for TNFalphapurchase
MBS355357 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TNFalpha, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TNFalpha. The ELISA analytical biochemical technique of the MBS355357 kit is based on TNFalpha antibody-TNFalpha antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TNFalpha antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TNFalpha. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TNFalpha elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- TNFalpha polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- TNFalpha polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TNFalpha amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TNFalpha can be calculated.

Background: Tumor necrosis factor, formerly known as tumor necrosis factor-alpha or TNF-alpha, is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. It was originally identified in Human serum after injection with Mycobacterium bovis strain bacillus Calmette-Guerin (BCG) and endotoxin. TNF is primarily produced as a 212-amino acid-long type II transmembrane protein arranged in stable homotrimers. The human TNF gene maps to chromosome 6p21.3, spans about 3 kilobases and contains 4 exons. The primary role of TNF is in the regulation of immune cells. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD).
Applications Tested/Suitable for TNFalpha elisa kit
Quantitative sELISA (EIA)
Application Notes for TNFalpha elisa kit
For quantitative detection of TNFα in Human serum, body fluids, tissue lysates or cell culture supernatants.

Typical Testing Data/Standard Curve (for reference only) of TNFalpha elisa kit
TNFalpha elisa kit  Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS355357. Click to request current manual
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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