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TNFRSF1A recombinant protein :: Tumor Necrosis Factor Receptor 1, Soluble Recombinant Protein

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Catalog # MBS650997
Unit / Price
  0.005 mg  /  $315 +1 FREE 8GB USB
  0.02 mg  /  $455 +1 FREE 8GB USB
TNFRSF1A recombinant protein
Product Name

Tumor Necrosis Factor Receptor 1, Soluble (TNFRSF1A), Recombinant Protein

Full Product Name

Tumor Necrosis Factor Receptor 1, Soluble, Recombinant, Human (sTNFR1)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MDSVCPQGKY IHPQNNSICC TKCHKGTYLY NDCPGPGQDT DCRECESGSF TASENHLRHC LSCSKCRKEM GQVEISSCTV DRDTVCGCRK NQYRHYWSEN LFQCFNCSLC LNGTVHLSCQ EKQNTVCTCH AGFFLRENEC VSCSNCKKSL ECTKLCLPQI EN
Chromosome Location
Chromosome: 12; NC_000012.11 (6437923..6451283, complement). Location: 12p13.2
OMIM
142680
3D Structure
ModBase 3D Structure for P19438
Purity/Purification
98% by SDS-PAGE and HPLC analyses.
Form/Format
Supplied as a lyophilized powder from 10mM Tris, pH 8.0, 20mM sodium chloride. Reconstitute in sterile 5mM Tris, pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex.
Biological Activity
Determined by its inhibitory effect of the TNF-alpha mediated cytotoxicity in murine L-929 cells. The ED50 for this effect in the presence of 0.25ng/ml of recombinant human TNF-alpha, is 0.05ug/ml.
Country of Origin
USA
Source
Recombinant corresponding to 162aa from the cysteine rich ligand binding portion of the extracellular domain of the human TNFR1 protein expressed in E. coli.
Endotoxin
0.1ng/ug (1EU/ug).
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with 5mM Tris, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of TNFRSF1A recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TNFRSF1A recombinant protein
TNFR1 belongs to the TNFR superfamily of transmembrane proteins, and is expressed in most cell types. Binding of either TNF-alpha or TNF-beta to TNFR1 initiates a signal transduction pathway that results in the activation of the transduction factor NFkappaB, whose target genes are involved in the regulation of inflammatory responses, and, in certain cells induce apoptosis. Soluble TNF Receptor I (sTNFR1) is capable of inhibiting TNF-alpha and TNF-beta activities by acting as a decoy receptor that serves as a sink for the TNF ligands. Recombinant human sTNFR1 is an 18.3kD protein (162 amino acid residues) comprising the cysteine rich ligand binding portion of the extracellular domain of the TNFR1 protein.
NCBI/Uniprot data below describe general gene information for TNFRSF1A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
50,495 Da[Similar Products]
NCBI Official Full Name
tumor necrosis factor receptor 1
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 1A
NCBI Official Symbol
TNFRSF1A  [Similar Products]
NCBI Official Synonym Symbols
FPF; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55; MGC19588
  [Similar Products]
NCBI Protein Information
tumor necrosis factor receptor superfamily member 1A; TNF-R1; TNF-RI; TNFR-I; OTTHUMP00000238438; OTTHUMP00000238440; OTTHUMP00000238441; tumor necrosis factor-alpha receptor; tumor necrosis factor receptor type 1; tumor necrosis factor binding protein 1; tumor necrosis factor receptor 1A isoform beta
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 1A
UniProt Synonym Protein Names
Tumor necrosis factor receptor 1; TNF-R1; Tumor necrosis factor receptor type I; TNF-RI; TNFR-I; p55; p60
UniProt Gene Name
TNFRSF1A  [Similar Products]
UniProt Synonym Gene Names
TNFAR; TNFR1  [Similar Products]
UniProt Entry Name
TNR1A_HUMAN
NCBI Summary for TNFRSF1A
The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of this receptor were found to be associated with the autosomal dominant periodic fever syndrome. The impaired receptor clearance is thought to be a mechanism of the disease. [provided by RefSeq]
UniProt Comments for TNFRSF1A
Function: Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.

Subunit structure: Binding of TNF to the extracellular domain leads to homotrimerization. The aggregated death domains provide a novel molecular interface that interacts specifically with the death domain of TRADD. Various TRADD-interacting proteins such as TRAFS, RIPK1 and possibly FADD, are recruited to the complex by their association with TRADD. This complex activates at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4, BRE, FEM1B, GRB2, SQSTM1 and TRPC4AP. Interacts with HCV core protein. Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18

Subcellular location: Cell membrane; Single-pass type I membrane protein. Secreted.

Domain: The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP

Post-translational modification: The soluble form is produced from the membrane form by proteolytic processing.

Involvement in disease: Defects in TNFRSF1A are the cause of familial hibernian fever (FHF) [

MIM:142680]; also known as tumor necrosis factor receptor-associated periodic syndrome (TRAPS). FHF is a hereditary periodic fever syndrome characterized by recurrent fever, abdominal pain, localized tender skin lesions and myalgia. Reactive amyloidosis is the main complication and occurs in 25% of cases. Ref.21 Ref.22 Ref.23 Ref.24 Ref.25

Sequence similarities: Contains 1 death domain.Contains 4 TNFR-Cys repeats.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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