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TNFSF15 elisa kit :: Mouse tumor necrosis factor (ligand) superfamily, member 15 ELISA Kit

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Catalog # MBS705929
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $435 +1 FREE 8GB USB
  96-Strip-Wells  /  $625 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,905 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

tumor necrosis factor (ligand) superfamily, member 15 (TNFSF15), ELISA Kit

Popular Item
Also Known As

Mouse tumor necrosis factor-like ligand 1, TL1 ELISA Kit

Product Synonym Names
Mouse tumor necrosis factor-like ligand 1 (TL1) ELISA kit; MGC129934; MGC129935; TL1; TL1A; VEGI; VEGI192A; TNF ligand-related molecule 1; TNF superfamily ligand TL1A; vascular endothelial cell growth inhibitor; vascular endothelial growth inhibitor-192A; tumor necrosis factor (ligand) superfamily; member 15
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS705929 Testing Data
Sequence Length
270
3D Structure
ModBase 3D Structure for Q5UBV8
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse TL1. No significant cross-reactivity or interference between mouse TL1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates.
Assay Type
Sandwich
Detection Range
62.5 pg/ml-4000 pg/ml.
Sensitivity
Typically less than 15.6 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TNFSF15 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTNFSF15purchase
MBS705929 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tumor necrosis factor (ligand) superfamily, member 15 (TNFSF15) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TNFSF15. The ELISA analytical biochemical technique of the MBS705929 kit is based on TNFSF15 antibody-TNFSF15 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TNFSF15 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TNFSF15. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). This product is a highly sensitive sandwich ELISA assay kit for detecting and quantifying mouse tumor necrosis factor ligand superfamily member 15 (TNFSF15). In this indirect mouse tumor necrosis factor-like ligand 1 (TL1) ELISA assay, known amounts of anti-TL1 antibodies are adsorbed to each plate well. A sample containing TL1 is added to each well and binds to capture antibodies. Unbound antigen is washed away. Subsequently, a detection antibody solution containing biotin conjugated anti TL1 antibodies, forming a complex with the previously bound TL1 and anti TL1 capture antibodies. Another wash removes unbound biotin conjugated antibodies, and avidin conjugated with horseradish peroxidase is added. A final wash removes any unbound avidin. Finally, a chromogenic substrate is added. The amount of bound enzyme varies directly with the concentration of protein in the original test sample, thus the absorbance at 450nm is inversely proportional with the concentration of mouse tumor necrosis factor-like ligand 1 added to each well. The concentration of TL1 in the test sample can be derived from a calibration curve of known standards.
Related Product Information for
TNFSF15 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of TNFSF15 elisa kit
TNFSF15 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS705929. Click to request current manual
NCBI/Uniprot data below describe general gene information for TNFSF15. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
27,723 Da
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 15
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 15
NCBI Official Symbol
Tnfsf15  [Similar Products]
NCBI Official Synonym Symbols
Tl1; Tl1a; Vegi
  [Similar Products]
NCBI Protein Information
tumor necrosis factor ligand superfamily member 15; TNF ligand-related molecule 1; vascular endothelial growth inhibitor; vascular endothelial cell growth inhibitor; bM20K13.3 (tumor necrosis factor (ligand) superfamily, member 15)
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 15
UniProt Synonym Protein Names
TNF ligand-related molecule 1; Vascular endothelial cell growth inhibitor
UniProt Gene Name
Tnfsf15  [Similar Products]
UniProt Synonym Gene Names
Tl1; Vegi  [Similar Products]
UniProt Entry Name
TNF15_MOUSE
UniProt Comments for TNFSF15
Function: Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis Promotes splenocyte alloactivation.1 PublicationManual assertion based on experiment in:Ref.1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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