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TNNI2 elisa kit :: Porcine TNNI2 ELISA Kit

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Catalog # MBS2514548
Unit / Price
  48-Strip-Wells  /  $450 +1 FREE 8GB USB
  96-Strip-Wells  /  $535 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,255 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,975 +4 FREE 8GB USB
TNNI2 elisa kit
Product Name

TNNI2, ELISA Kit

Popular Item
Also Known As

Porcine TNNI2 (Troponin I Type 2, Fast Skeletal) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2514548 COA
Sequence Length
182
OMIM
191043
3D Structure
ModBase 3D Structure for P48788
Species Reactivity
Specificity
This kit recognizes natural and recombinant Porcine TNNI2. No significant cross-reactivity or interference between Porcine TNNI2 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
78.125-5000pg/mL
Sensitivity
Min: 46.875pg/mL; Max: 5000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TNNI2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTNNI2purchase
MBS2514548 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TNNI2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TNNI2. The ELISA analytical biochemical technique of the MBS2514548 kit is based on TNNI2 antibody-TNNI2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TNNI2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TNNI2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TNNI2 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine TNNI2 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TNNI2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TNNI2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TNNI2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TNNI2. You can calculate the concentration of TNNI2 in the samples by comparing the OD of the samples to the standard curve.
Sample Manual Insert of MBS2514548. Click to request current manual
NCBI/Uniprot data below describe general gene information for TNNI2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21,355 Da
NCBI Official Full Name
troponin I, fast skeletal muscle isoform 2
NCBI Official Synonym Full Names
troponin I type 2 (skeletal, fast)
NCBI Official Symbol
TNNI2  [Similar Products]
NCBI Official Synonym Symbols
DA2B; FSSV; fsTnI; AMCD2B
  [Similar Products]
NCBI Protein Information
troponin I, fast skeletal muscle; troponin I fast twitch 2; troponin I, fast-twitch isoform; troponin I, fast-twitch skeletal muscle isoform; troponin I, skeletal, fast
UniProt Protein Name
Troponin I, fast skeletal muscle
UniProt Synonym Protein Names
Troponin I, fast-twitch isoform
Protein Family
UniProt Gene Name
TNNI2  [Similar Products]
UniProt Entry Name
TNNI2_HUMAN
NCBI Summary for TNNI2
This gene encodes a fast-twitch skeletal muscle protein, a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible for the calcium-dependent regulation of striated muscle contraction. Mouse studies show that this component is also present in vascular smooth muscle and may play a role in regulation of smooth muscle function. In addition to muscle tissues, this protein is found in corneal epithelium, cartilage where it is an inhibitor of angiogenesis to inhibit tumor growth and metastasis, and mammary gland where it functions as a co-activator of estrogen receptor-related receptor alpha. This protein also suppresses tumor growth in human ovarian carcinoma. Mutations in this gene cause myopathy and distal arthrogryposis type 2B. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]
UniProt Comments for TNNI2
TNNI2: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Defects in TNNI2 are a cause of distal arthrogryposis type 2B (DA2B); also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. Belongs to the troponin I family.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: troponin complex; cytosol; nucleus

Molecular Function: troponin T binding; protein binding; actin binding

Biological Process: skeletal muscle contraction; regulation of muscle contraction; positive regulation of transcription, DNA-dependent; muscle filament sliding

Disease: Arthrogryposis, Distal, Type 2b
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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