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TNNI3 recombinant protein :: Troponin I Type 3, Cardiac (TNNI3) Recombinant Protein

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Catalog # MBS2010502 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
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  0.01 mg  /  $130 +1 FREE 8GB USB
  0.05 mg  /  $225 +1 FREE 8GB USB
  0.1 mg  /  $340 +1 FREE 8GB USB
  0.2 mg  /  $415 +1 FREE 8GB USB
  0.5 mg  /  $800 +1 FREE 8GB USB
  1 mg  /  $1,180 +1 FREE 8GB USB
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Product Name

Troponin I Type 3, Cardiac (TNNI3), Recombinant Protein

Popular Item
Full Product Name

Recombinant Troponin I Type 3, Cardiac (TNNI3)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 19; NC_000019.9 (55663135..55669100, complement). Location: 19q13.4
OMIM
115210
3D Structure
ModBase 3D Structure for P19429
Host
E. coli
Species Reactivity
Human
Purity/Purification
>98%
Form/Format
10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
Concentration
200µg/mL (lot specific)
Source
Prokaryotic expression
Residues
Met1~Gly203
Tags
N-terminal His-Tag
Tissue Specificity
Skeletal Muscle
Traits
Freeze-dried powder
Predicted isoelectric point
9.7
Predicted Molecular Mass
24.4kDa
Accurate Molecular Mass
28kDa as determined by SDS-PAGE reducing conditions
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of TNNI3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for TNNI3 recombinant protein
Positive Control, Immunogen, SDS-PAGE, WB.

(May be suitable for use in other assays to be determined by the end user.)

NCBI/Uniprot data below describe general gene information for TNNI3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
troponin I, cardiac muscle
NCBI Official Synonym Full Names
troponin I type 3 (cardiac)
NCBI Official Symbol
TNNI3  [Similar Products]
NCBI Official Synonym Symbols
CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
  [Similar Products]
NCBI Protein Information
troponin I, cardiac muscle
UniProt Protein Name
Troponin I, cardiac muscle
UniProt Synonym Protein Names
Cardiac troponin I
Protein Family
UniProt Gene Name
TNNI3  [Similar Products]
UniProt Synonym Gene Names
TNNC1  [Similar Products]
UniProt Entry Name
TNNI3_HUMAN
NCBI Summary for TNNI3
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]
UniProt Comments for TNNI3
TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family.

Protein type: Motility/polarity/chemotaxis; Motor; Actin-binding

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: sarcomere; troponin complex; cytosol

Molecular Function: troponin T binding; protein domain specific binding; troponin C binding; protein binding; metal ion binding; calcium channel inhibitor activity; actin binding; protein kinase binding; calcium-dependent protein binding

Biological Process: cellular calcium ion homeostasis; regulation of smooth muscle contraction; heart contraction; regulation of systemic arterial blood pressure by ischemic conditions; heart development; ventricular cardiac muscle morphogenesis; vasculogenesis; negative regulation of ATPase activity; muscle filament sliding; cardiac muscle contraction

Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Familial Restrictive, 1; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Dilated, 2a
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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