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TNNI3 elisa kit :: Mouse troponin I type 3 (cardiac) ELISA Kit

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Catalog # MBS703536
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Typical Testing Data/Standard Curve (for reference only)
Product Name

troponin I type 3 (cardiac) (TNNI3), ELISA Kit

Popular Item
Full Product Name

Mouse cardiac troponin I , cTn-I ELISA Kit

Product Synonym Names
Mouse cardiac troponin I (cTn-I) ELISA kit; CMD2A; CMH7; MGC116817; RCM1; TNNC1; cTnI; familial hypertrophic cardiomyopathy 7; troponin I; cardiac; troponin I type 3 (cardiac)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS703536 Testing Data
3D Structure
ModBase 3D Structure for Q6FGX2
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse cTn-I. No significant cross-reactivity or interference between mouse cTn-I and analogues was observed.
Samples
Serum, plasma
Detection Range
15.6 pg/ml -1000 pg/mL
Sensitivity
<3.9 pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TNNI3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for TNNI3purchase
MBS703536 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the troponin I type 3 (cardiac) (TNNI3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TNNI3. The ELISA analytical biochemical technique of the MBS703536 kit is based on TNNI3 antibody-TNNI3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TNNI3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TNNI3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TNNI3 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for cTn-I has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any cTn-I present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for cTn-I is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of cTn-I bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of TNNI3 elisa kit
TNNI3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS703536. Click to request current manual
NCBI/Uniprot data below describe general gene information for TNNI3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
24,008 Da
NCBI Official Full Name
TNNI3
NCBI Official Synonym Full Names
troponin I type 3 (cardiac)
NCBI Official Symbol
TNNI3  [Similar Products]
NCBI Official Synonym Symbols
CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
  [Similar Products]
NCBI Protein Information
troponin I, cardiac muscle; cardiomyopathy, dilated 2A (autosomal recessive)
UniProt Protein Name
TNNI3 protein
UniProt Synonym Protein Names
TNNI3 protein; Troponin I type 3 (Cardiac); Troponin I, cardiac; Troponin I, cardiac variant
Protein Family
UniProt Gene Name
TNNI3  [Similar Products]
UniProt Entry Name
Q6FGX2_HUMAN
NCBI Summary for TNNI3
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]
UniProt Comments for TNNI3
TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family.

Protein type: Motor; Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: sarcomere; troponin complex; cytosol

Molecular Function: troponin T binding; troponin C binding; protein domain specific binding; protein binding; metal ion binding; calcium channel inhibitor activity; actin binding; calcium-dependent protein binding; protein kinase binding

Biological Process: cellular calcium ion homeostasis; regulation of smooth muscle contraction; heart contraction; regulation of systemic arterial blood pressure by ischemic conditions; heart development; ventricular cardiac muscle morphogenesis; vasculogenesis; negative regulation of ATPase activity; cardiac muscle contraction; muscle filament sliding

Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Familial Restrictive, 1; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Dilated, 2a
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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