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TNNT1 elisa kit :: Human Troponin T, slow skeletal muscle ELISA Kit

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Catalog # MBS289077
Unit / Price
  48-Strip-Wells  /  $355 +1 FREE 8GB USB
  96-Strip-Wells  /  $475 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,030 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,700 +4 FREE 8GB USB
 Typical Testing Data/Standard Curve (for reference only)
Product Name

Troponin T, slow skeletal muscle (TNNT1), ELISA Kit

Popular Item
Also Known As

Human Troponin T, slow skeletal muscle ELISA Kit

Product Synonym Names
Troponin T, slow skeletal muscle; TnTs; Slow skeletal muscle troponin T; sTnT; TNNT1; TNT
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS289077 Testing Data
Sequence Length
262
OMIM
191041
3D Structure
ModBase 3D Structure for P13805
Species Reactivity
Specificity
Recombinant and natural Human Troponin T, slow skeletal muscle,TnTs
Samples
Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
Detection Range
15.6-1000 pg/mL
Sensitivity
<8.9 pg/mL
Intra-assay Precision
Intra-Assay CV: <=4.9%
Inter-assay Precision
Inter-Assay CV: <=7.7%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TNNT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTNNT1purchase
MBS289077 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Troponin T, slow skeletal muscle (TNNT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TNNT1. The ELISA analytical biochemical technique of the MBS289077 kit is based on TNNT1 antibody-TNNT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TNNT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TNNT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TNNT1 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of TNNT1 elisa kit
TNNT1 elisa kit  Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS289077. Click to request current manual
NCBI/Uniprot data below describe general gene information for TNNT1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
31,242 Da
NCBI Official Full Name
troponin T, slow skeletal muscle isoform b
NCBI Official Synonym Full Names
troponin T1, slow skeletal type
NCBI Official Symbol
TNNT1  [Similar Products]
NCBI Official Synonym Symbols
ANM; TNT; NEM5; STNT; TNTS
  [Similar Products]
NCBI Protein Information
troponin T, slow skeletal muscle
UniProt Protein Name
Troponin T, slow skeletal muscle
UniProt Synonym Protein Names
Slow skeletal muscle troponin T; sTnT
Protein Family
UniProt Gene Name
TNNT1  [Similar Products]
UniProt Synonym Gene Names
TNT; TnTs; sTnT  [Similar Products]
UniProt Entry Name
TNNT1_HUMAN
NCBI Summary for TNNT1
This gene encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for TNNT1
TNNT1: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Defects in TNNT1 are the cause of nemaline myopathy type 5 (NEM5); also known as Amish nemaline myopathy (ANM). A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 5 is a severe and progressive form common among Old Order Amish. Affected infants display tremors with hypotonia and mild contractures of the shoulders and hips. Proximal contractures progressively weaken and a pectus carinatum deformity develops before children die of respiratory insufficiency, usually in the second year. Belongs to the troponin T family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Contractile

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: cytosol; troponin complex

Molecular Function: protein binding; tropomyosin binding; troponin T binding

Biological Process: muscle filament sliding; negative regulation of muscle contraction; skeletal muscle contraction; slow-twitch skeletal muscle fiber contraction; transition between fast and slow fiber

Disease: Nemaline Myopathy 5
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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