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TP elisa kit :: Human Thymidine phosphorylase ELISA Kit

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Catalog # MBS731022
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
TP elisa kit
Product Name

Thymidine phosphorylase (TP), ELISA Kit

Popular Item
Also Known As

Human Thymidine phosphorylase ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 603041
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
2.5-50ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTPpurchase
MBS731022 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Thymidine phosphorylase (TP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TP. The ELISA analytical biochemical technique of the MBS731022 kit is based on TP antibody-TP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TP elisa kit
Intended Uses: ELISA classic Toxoplasma gondii IgG and IgM tests are quantitative and qualitative immunoassays for the detection of human antibodies in serum or plasma directed against Toxoplasma gondii. The assays are recommended to determine the immune status and to differentiate acute from recent infections. The ELISA classic Toxoplasma gondii IgM test is the initial assay for the detection of acute infections. IgM antibodies directed against Toxoplasma gondii might persist over a long time period. ELISA classic Toxoplasma gondii IgG test in combination with ELISA classic Toxoplasma gondii avidity reagent (Order No.: B110AVID) allows the determination of IgG antibody avidity for the differentiation of acute from recent infections. ELISA classic Toxoplasma gondii IgM test is suitable for newborn screening with dried blood spots (DBS).

Principle of the Assay: To determine the performance characteristics of the ELISA classic Toxoplasma gondii IgG test, an external comparison study, utilizing sera from 450 patients and healthy individuals, was performed in parallel with an indirect immunofluorescence test. The results indicate a sensitivity of 98.2 % and specificity of 99.4 %. An internal study was also carried out using sera from 1000 pregnant women. An indirect immunofluorescence test was also used as the reference test and discrepant results were further tested in the Sabin-Feldman-Test to determine their true status. The results indicate a sensitivity of 97.8 % and specificity of 99.8 %. An external study, to determine the sensitivity and specificity of the ELISA classic Toxoplasma gondii IgM test, utilizing a total of 256 sera, of which 104 were positive, was carried out using two commercially available IgM tests as comparison ( from company A and company B). The positive IgM sera were selected using the Toxoplasma IgM test from company A. The use of a borderline region between 450 and 540 U/ml resulted in 19 positive sera not returning a positive result, seven of which were potentially from acute infections. In this evaluation the ELISA classic Toxoplasma gondii IgM test demonstrated a higher sensitivity than the test from company B which had returned negative test results for 28 of the 104 positive. Using the 450 and 540 U/ml borderline region the ELISA classic test when compared to that from company A returned a specificity of 98.6 %. Using a borderline region between 300 and 350 U/ml resulted in eleven positive sera not being recognized. Taking the results of further testing and the patientsÂ’ clinical background into account, only two of these discrepant sera were from possibly primary infections and the remaining 9 were the result of persisting IgM or unspecific IgM. In relation to diagnostically relevant primary infections a sensitivity of 97.8 % was calculated. A specificity of 95.7 % was determined in comparison to the ELISA from company A. The author of the external study determined the highest efficiency of the ELI SA classic Toxoplasma gondii IgM test to be attained when using the 300 to 350 U/ml borderline region. In an internal performance study to determine the sensitivity of the Toxoplasma gondii IgM a serum panel of 86 sera (29 of which were positive for IgM) was tested in the test and a further 2 commercially available IgM ELISA tests (company C and company B). the positive IgM sera were selected using the test from company C. Using a borderline region from 450 to 540 U/ml, five positive sera were not recognized so giving a sensitivity compared to the test from company C of 82.8 %. Compared to the test from company B the test recorded 100% sensitivity. To determine the specificity 372 sera from pregnant women and blood donors were investigated. Using the same borderline region from 450 to 540 U/ml a specificity of 98.9 % compared to the test from company B was attained. Borderline results were not included in the calculations. ELISA classic Toxoplasma gondii IgG test is suitable for the determination of immune status in pregnant women. Hemoglobin levels of up to 10 mg/ml in serum had no effect on the results obtained. Investigation of a serum panel with antibody activities against potentially cross-reacting parameters (sera with auto-antibodies, rheumatoid factor, EBV and syphilis sera) did not reveal any evidence of false positive results due to cross-reactions. Infection with Epstein-Barr-Virus may lead to a polyclonal stimulation of antibody production and so lead to false positive Toxoplasma gondii IgM antibody results. In addition it should be noted that Toxoplasma gondii igM antibodies may persist for months or even years after an infection has resolved.
Sample Manual Insert of MBS731022. Click to request current manual
NCBI/Uniprot data below describe general gene information for TP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
50,405 Da
NCBI Official Full Name
thymidine phosphorylase isoform 1 proprotein
NCBI Official Synonym Full Names
thymidine phosphorylase
NCBI Official Symbol
NCBI Official Synonym Symbols
TP; ECGF; ECGF1; MNGIE; MEDPS1; MTDPS1; PDECGF; hPD-ECGF
  [Similar Products]
NCBI Protein Information
thymidine phosphorylase; tdRPase; gliostatin
UniProt Protein Name
Thymidine phosphorylase
UniProt Synonym Protein Names
Gliostatin; Platelet-derived endothelial cell growth factor; PD-ECGF; TdRPase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
ECGF1; TP; PD-ECGF  [Similar Products]
UniProt Entry Name
TYPH_HUMAN
NCBI Summary for TP
This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012]
UniProt Comments for TP
ECGF1: May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro. Homodimer. Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.

Protein type: EC 2.4.2.4; DNA replication; Phosphorylase; Transferase; Xenobiotic Metabolism - drug metabolism - other enzymes; Cytokine; Nucleotide Metabolism - pyrimidine; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: cytosol

Molecular Function: thymidine phosphorylase activity; growth factor activity; phosphorylase activity; transferase activity, transferring pentosyl groups; pyrimidine-nucleoside phosphorylase activity; platelet-derived growth factor receptor binding

Biological Process: pyrimidine nucleoside catabolic process; mitochondrial genome maintenance; pyrimidine base metabolic process; pyrimidine nucleotide metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside salvage; angiogenesis; DNA replication; chemotaxis; cell differentiation

Disease: Mitochondrial Dna Depletion Syndrome 1 (mngie Type)
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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