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TP63 elisa kit :: Mouse tumor protein p63 ELISA Kit

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Catalog # MBS934073
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Typical Testing Data/Standard Curve (for reference only)
Product Name

tumor protein p63 (TP63), ELISA Kit

Popular Item
Also Known As

Mouse Tumor protein 63, TP63 ELISA Kit

Product Synonym Names
Mouse Tumor protein 63 (TP63) ELISA kit; AIS; B (p51A); B (p51B); EEC3; KET; LMS; NBP; OFC8; RHS; SHFM4; TP53CP; TP53L; TP73L; p40; p51; p53CP; p63; p73H; p73L; OTTHUMP00000209738; OTTHUMP00000209740; amplified in squamous cell carcinoma; chron; tumor protein p63
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS934073 Testing Data
Sequence Length
680
3D Structure
ModBase 3D Structure for O88898
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse TP63. No significant cross-reactivity or interference between mouse TP63 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
25 pg/ml-1600 pg/ml.
Sensitivity
The minimum detectable dose of mouse TP63 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TP63 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTP63purchase
MBS934073 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tumor protein p63 (TP63) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TP63. The ELISA analytical biochemical technique of the MBS934073 kit is based on TP63 antibody-TP63 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TP63 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TP63. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TP63 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TP63 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TP63 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TP63 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TP63 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of TP63 elisa kit
TP63 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS934073. Click to request current manual
NCBI/Uniprot data below describe general gene information for TP63. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
76,789 Da
NCBI Official Full Name
tumor protein 63 isoform a
NCBI Official Synonym Full Names
transformation related protein 63
NCBI Official Symbol
Trp63  [Similar Products]
NCBI Official Synonym Symbols
Ket; P63; P73l; Tp63; P51/P63; AI462811; Trp53rp1
  [Similar Products]
NCBI Protein Information
tumor protein 63; transformation-related protein 63
UniProt Protein Name
Tumor protein 63
UniProt Synonym Protein Names
Transformation-related protein 63; TP63; Tumor protein p73-like; p73L
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
P63; P73l; Tp73l; Trp63; p63; TP63; p73L  [Similar Products]
UniProt Entry Name
P63_MOUSE
NCBI Summary for TP63
This gene encodes tumor protein p63, a member of the p53 family of transcription factors involved in cellular responses to stress and development. The family members include tumor proteins p53, p63, and p73, which have high sequence similarity to one another. This similarity allows p63 and p73 to transactivate p53-responsive genes causing cell cycle arrest and apoptosis. The family members can interact with each other in many ways, including direct and indirect protein interactions. This results in mutual regulation of target gene promoters. Tumor protein p63 -/- mice have several developmental defects which include the lack of limbs and other tissues, such as teeth and mammary glands, which develop as a result of interactions between mesenchyme and epithelium. Both alternative splicing and the use of alternative promoters result in multiple transcript variants encoding different protein isoforms.[provided by RefSeq, Dec 2009]
UniProt Comments for TP63
p63: Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter. Binds DNA as a homotetramer. Isoform composition of the tetramer may determine transactivation activity. Isoforms Alpha and Gamma interact with HIPK2. Interacts with SSRP1, leading to stimulate coactivator activity. Isoform 1 and isoform 2 interact with WWP1. Interacts with PDS5A. Isoform 5 (via activation domain) interacts with NOC2L. Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues. Belongs to the p53 family. 12 isoforms of the human protein are produced by alternative promoter.

Protein type: Transcription factor; DNA-binding

Cellular Component: nucleoplasm; transcription factor complex; neuron projection; protein complex; rough endoplasmic reticulum; cytoplasm; dendrite; nuclear chromatin; intracellular; cytosol; chromatin; nucleus

Molecular Function: protein domain specific binding; identical protein binding; protein binding; DNA binding; p53 binding; sequence-specific DNA binding; metal ion binding; double-stranded DNA binding; damaged DNA binding; WW domain binding; chromatin binding; transcription factor activity

Biological Process: prostate gland development; multicellular organismal development; positive regulation of transcription, DNA-dependent; protein homotetramerization; hair follicle development; positive regulation of mesenchymal cell proliferation; regulation of neuron apoptosis; epithelial cell development; anatomical structure formation; morphogenesis of a polarized epithelium; response to X-ray; embryonic limb morphogenesis; urinary bladder development; skin morphogenesis; transcription, DNA-dependent; negative regulation of keratinocyte differentiation; cell aging; keratinocyte differentiation; organ morphogenesis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; G1 DNA damage checkpoint; transcription from RNA polymerase II promoter; epidermis development; ectoderm and mesoderm interaction; apoptosis; cloacal septation; epidermal cell division; negative regulation of transcription from RNA polymerase II promoter; regulation of caspase activity; polarized epithelial cell differentiation; smooth muscle development; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; sympathetic nervous system development; epithelial cell differentiation; regulation of transcription, DNA-dependent; response to gamma radiation; establishment of planar polarity; cell differentiation; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; skeletal development; female genitalia morphogenesis; positive regulation of Notch signaling pathway; protein tetramerization; proximal/distal pattern formation; Notch signaling pathway; regulation of epidermal cell division; hair follicle morphogenesis; multicellular organismal aging; keratinocyte proliferation; replicative cell aging; pattern specification process; odontogenesis of dentine-containing teeth; chromatin remodeling; positive regulation of osteoblast differentiation; neuron apoptosis; response to DNA damage stimulus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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