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TPH1 elisa kit :: Rat Tryptophan Hydroxylase 1 ELISA Kit

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Catalog # MBS2504597
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Tryptophan Hydroxylase 1 (TPH1), ELISA Kit

Popular Item
Full Product Name

Rat Tryptophan Hydroxylase 1(TPH1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
191060
3D Structure
ModBase 3D Structure for P17752
Species Reactivity
Specificity
This kit recognizes natural and recombinant Rat TPH1. No significant cross-reactivity or interference between Rat TPH1 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.313-20ng/mL
Sensitivity
Min: 0.188ng/mL; Max: 20ng/mL
Intended Uses
This ELISA kit applies to the in vitro quantitative determination of Rat TPH1 concentrations in serum, plasma and other biological fluids.
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TPH1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for TPH1purchase
MBS2504597 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Tryptophan Hydroxylase 1 (TPH1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TPH1. The ELISA analytical biochemical technique of the MBS2504597 kit is based on TPH1 antibody-TPH1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TPH1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TPH1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TPH1 elisa kit
Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TPH1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TPH1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TPH1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TPH1. You can calculate the concentration of TPH1 in the samples by comparing the OD of the samples to the standard curve. FOR

Typical Testing Data/Standard Curve (for reference only) of TPH1 elisa kit
TPH1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2504597. Click to request current manual
NCBI/Uniprot data below describe general gene information for TPH1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
53,394 Da
NCBI Official Full Name
Tryptophan hydroxylase 1
NCBI Official Synonym Full Names
tryptophan hydroxylase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
TPRH; TRPH
  [Similar Products]
NCBI Protein Information
tryptophan 5-hydroxylase 1; L-tryptophan hydroxylase; indoleacetic acid-5-hydroxylase; tryptophan 5-monooxygenase 1; tryptophan hydroxylase (tryptophan 5-monooxygenase)
UniProt Protein Name
Tryptophan 5-hydroxylase 1
UniProt Synonym Protein Names
Tryptophan 5-monooxygenase 1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
TPH; TPRH; TRPH  [Similar Products]
UniProt Entry Name
TPH1_HUMAN
NCBI Summary for TPH1
This gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.[provided by RefSeq, Apr 2009]
UniProt Comments for TPH1
TPH1: an enzyme that is rate-limiting in the biosynthesis of serotonin. It catalyzes the biopterin dependent monooxygenation of tryptophan to 5-hydroxytryptophan (5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. Its expression is limited to a few specialized tissues: raphe neurons, pinealocytes, mast cells, mononuclear leukocytes, beta-cells of the islets of Langerhans, and intestinal and pancreatic enterochromaffin cells. Two alternatively spliced isoforms have been described.

Protein type: Oxidoreductase; Amino Acid Metabolism - tryptophan; EC 1.14.16.4

Chromosomal Location of Human Ortholog: 11p15.3-p14

Cellular Component: neuron projection; cytosol

Molecular Function: amino acid binding; tryptophan 5-monooxygenase activity; iron ion binding

Biological Process: circadian rhythm; indolalkylamine biosynthetic process; aromatic amino acid family metabolic process; negative regulation of ossification; positive regulation of fat cell differentiation; bone remodeling; serotonin biosynthetic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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