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TPI1 elisa kit :: Human triosephosphate isomerase 1 ELISA Kit

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Catalog # MBS901437
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Typical Testing Data/Standard Curve (for reference only)
Product Name

triosephosphate isomerase 1 (TPI1), ELISA Kit

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Full Product Name

Human Triosephosphate isomerase, TPI1 ELISA Kit

Product Synonym Names
Human Triosephosphate isomerase (TPI1) ELISA kit; MGC88108; TPI; ; triosephosphate isomerase 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS901437 Testing Data
OMIM
gene 615512
3D Structure
ModBase 3D Structure for P60174
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human TPI1. No significant cross-reactivity or interference between human TPI1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates.
Assay Type
Sandwich
Detection Range
28 pg/ml -1800 pg/ml.
Sensitivity
7 pg /ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TPI1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for TPI1purchase
MBS901437 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the triosephosphate isomerase 1 (TPI1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TPI1. The ELISA analytical biochemical technique of the MBS901437 kit is based on TPI1 antibody-TPI1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TPI1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TPI1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TPI1 elisa kit
Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TPI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPI1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of TPI1 elisa kit
TPI1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS901437. Click to request current manual
NCBI/Uniprot data below describe general gene information for TPI1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
30,791 Da
NCBI Official Full Name
triosephosphate isomerase isoform 1
NCBI Official Synonym Full Names
triosephosphate isomerase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
TIM; TPI; TPID; HEL-S-49
  [Similar Products]
NCBI Protein Information
triosephosphate isomerase; triose-phosphate isomerase; epididymis secretory protein Li 49
UniProt Protein Name
Triosephosphate isomerase
UniProt Synonym Protein Names
Triose-phosphate isomerase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
TPI; TIM  [Similar Products]
UniProt Entry Name
TPIS_HUMAN
NCBI Summary for TPI1
This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
UniProt Comments for TPI1
TPI1: Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Belongs to the triosephosphate isomerase family. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Carbohydrate Metabolism - inositol phosphate; EC 5.3.1.1; Isomerase; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: extracellular space; cytosol; nucleus

Molecular Function: protein binding; triose-phosphate isomerase activity

Biological Process: pentose-phosphate shunt; glycolysis; multicellular organismal development; carbohydrate metabolic process; glucose metabolic process; pathogenesis; gluconeogenesis

Disease: Triosephosphate Isomerase Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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