• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

TPSb2 recombinant protein :: Tryptase Beta 2 Recombinant Protein

Scan QR to view Datasheet
Catalog # MBS2031483 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $145 +1 FREE 8GB USB
  0.05 mg  /  $285 +1 FREE 8GB USB
  0.1 mg  /  $445 +1 FREE 8GB USB
  0.2 mg  /  $545 +1 FREE 8GB USB
  0.5 mg  /  $1,055 +1 FREE 8GB USB
  1 mg  /  $1,575 +1 FREE 8GB USB
Product Name

Tryptase Beta 2 (TPSb2), Recombinant Protein

Popular Item
Also Known As

Recombinant Tryptase Beta 2 (TPSb2)

Matching Pairs
Matching Pairs
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
191081
Host
E. coli
Species Reactivity
Rat
Purity/Purification
>98%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
Concentration
200ug/mL (lot specific)
Source
Prokaryotic expression
Residues
Ile30~Ser274
Tags
N-terminal His-Tag
Subcellular Location
Secreted
Traits
Freeze-dried powder
Predicted isoelectric point
5.9
Predicted Molecular Mass
31.2kDa
Accurate Molecular Mass
35kDa as determined by SDS-PAGE reducing conditions
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Other Notes
Small volumes of TPSb2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for TPSb2 recombinant protein
SDS-PAGE, WB, ELISA, IP, CoIP, Purification, Amine Reactive Labeling.

(May be suitable for use in other assays to be determined by the end user.)

Sequence Information of TPSb2 recombinant protein
TPSb2 recombinant protein Sequence Information image
TPSb2 recombinant protein Testing Data image
NCBI/Uniprot data below describe general gene information for TPSb2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
tryptase beta-2 preproprotein
NCBI Official Synonym Full Names
tryptase beta 2 (gene/pseudogene)
NCBI Official Symbol
TPSB2  [Similar Products]
NCBI Official Synonym Symbols
TPS2; tryptaseB; tryptaseC
  [Similar Products]
NCBI Protein Information
tryptase beta-2
UniProt Protein Name
Tryptase beta-2
UniProt Synonym Protein Names
Tryptase II
Protein Family
UniProt Gene Name
TPSB2  [Similar Products]
UniProt Synonym Gene Names
TPS2; Tryptase-2  [Similar Products]
UniProt Entry Name
TRYB2_HUMAN
NCBI Summary for TPSb2
Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, beta II and beta III. Beta tryptases appear to be the main isoenzymes expressed in mast cells, whereas in basophils, alpha-tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]
UniProt Comments for TPSb2
TPSB2: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Has an immunoprotective role during bacterial infection. Required to efficiently combat K.pneumoniae infection. Belongs to the peptidase S1 family. Tryptase subfamily.

Protein type: Secreted, signal peptide; Secreted; EC 3.4.21.59; Protease

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: extracellular region

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: proteolysis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000