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TRACP-5b elisa kit :: Mouse tartrate resistant acid phosphatase 5b (TRACP-5b) ELISA Kit

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Catalog # MBS267963
Unit / Price
  48-Strip-Wells  /  $275 +1 FREE 8GB USB
  96-Strip-Wells  /  $435 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

tartrate resistant acid phosphatase 5b (TRACP-5b), ELISA Kit

Popular Item
Also Known As

Mouse tartrate resistant acid phosphatase 5b (TRACP-5b) ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
325
OMIM
gene 607944
Species Reactivity
Specificity
No cross-reaction with other factors.
Samples
Mouse serum, plasma or cell culture supernatant
Detection Range
50 U/L-0.78 U/L
Sensitivity
0.08 U/L
Intra-assay Precision
? 8%
Inter-assay Precision
? 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TRACP-5b elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTRACP-5bpurchase
MBS267963 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tartrate resistant acid phosphatase 5b (TRACP-5b) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TRACP-5b. The ELISA analytical biochemical technique of the MBS267963 kit is based on TRACP-5b antibody-TRACP-5b antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TRACP-5b antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TRACP-5b. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TRACP-5b elisa kit
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse TRACP-5b monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve (for reference only) of TRACP-5b elisa kit
TRACP-5b elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS267963. Click to request current manual
NCBI/Uniprot data below describe general gene information for TRACP-5b. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
36,599 Da
NCBI Official Full Name
tartrate-resistant acid phosphatase type 5
NCBI Official Synonym Full Names
acid phosphatase 5, tartrate resistant
NCBI Official Symbol
NCBI Official Synonym Symbols
TRAP; SPENCDI
  [Similar Products]
NCBI Protein Information
tartrate-resistant acid phosphatase type 5; TrATPase; tartrate-resistant acid ATPase
UniProt Protein Name
Tartrate-resistant acid phosphatase type 5
UniProt Synonym Protein Names
Tartrate-resistant acid ATPase; TrATPase; Type 5 acid phosphatase
UniProt Gene Name
UniProt Synonym Gene Names
TR-AP; TrATPase  [Similar Products]
UniProt Entry Name
PPA5_HUMAN
NCBI Summary for TRACP-5b
This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq, Aug 2008]
UniProt Comments for TRACP-5b
ACP5: Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. Defects in ACP5 are the cause of spondyloenchondrodysplasia with immune dysregulation (SPENCDI). A disease characterized by vertebral and metaphyseal dysplasia, spasticity with cerebral calcifications, and strong predisposition to autoimmune diseases. The skeletal dysplasia is characterized by radiolucent and irregular spondylar and metaphyseal lesions that represent islands of chondroid tissue within bone. ACP5 inactivating mutations result in a functional excess of phosphorylated osteopontin causing deregulation of osteopontin signaling and consequential autoimmune disease. Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

Protein type: EC 3.1.3.2; Cofactor and Vitamin Metabolism - riboflavin; Motility/polarity/chemotaxis; Phosphatase

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: lysosome; integral to membrane; cytosol

Molecular Function: acid phosphatase activity; ferric iron binding; ferrous iron binding

Biological Process: negative regulation of interleukin-12 production; vitamin metabolic process; negative regulation of nitric oxide biosynthetic process; response to lipopolysaccharide; negative regulation of superoxide release; negative regulation of tumor necrosis factor production; negative regulation of interleukin-1 beta production; riboflavin metabolic process; defense response to Gram-positive bacterium; dephosphorylation; response to cytokine stimulus; negative regulation of inflammatory response; water-soluble vitamin metabolic process; bone resorption

Disease: Spondyloenchondrodysplasia With Immune Dysregulation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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