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TRAP1 elisa kit :: Human TNF receptor-associated protein 1 ELISA Kit

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Catalog # MBS706000
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $550 +1 FREE 8GB USB
  96-Strip-Wells  /  $795 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

TNF receptor-associated protein 1 (TRAP1), ELISA Kit

Popular Item
Full Product Name

Human Heat shock protein 75 kDa, mitochondrial, TRAP1/HSP75 ELISA Kit

Product Synonym Names
Human Heat shock protein 75 kDa; mitochondrial (TRAP1/HSP75) ELISA kit; HSP75; HSP90L; heat shock protein 75; tumor necrosis factor type 1 receptor associated protein; TNF receptor-associated protein 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS706000 Testing Data
OMIM
606219
3D Structure
ModBase 3D Structure for Q12931
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human TRAP1. No significant cross-reactivity or interference between human TRAP1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
0.6 25 ng/ml -40 ng/ml.
Sensitivity
The minimum detectable dose of human TRAP1 is typically less than 0.156 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TRAP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTRAP1purchase
MBS706000 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TNF receptor-associated protein 1 (TRAP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TRAP1. The ELISA analytical biochemical technique of the MBS706000 kit is based on TRAP1 antibody-TRAP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TRAP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TRAP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TRAP1 - heat shock protein 75 - is a mitochondrial localized homolog of HSP 90. Publications have shown this protein interacts with tumor necrosis factor like receptor and the transcription factor Rb activating inflammatory pathways. Quantitation of cell samples or large sample arrays are made simple with a TRAP 1 ELISA assay. Available in pre-coated multi wells (48) or micrometer plate kits, these ELISA kits show excellent sensitivity and dynamic range for detection of human HSP 75 kDa. All required reagents are provided in this product, including primary antibody, secondary antibodies, wash products and proteins for standard curve generation. Because of high homology within the TNF receptor factor family, such as TNF receptor protein, soluble TNF receptor and TNF receptor II, any assay kit for mitochondrial TRAP1 requires the high level of specificity and lack of cross reaction available in this TRAP1 ELISA kit.
Related Product Information for
TRAP1 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TRAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRAP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of TRAP1 elisa kit
TRAP1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS706000. Click to request current manual
NCBI/Uniprot data below describe general gene information for TRAP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
80,110 Da
NCBI Official Full Name
heat shock protein 75 kDa, mitochondrial isoform 2
NCBI Official Synonym Full Names
TNF receptor-associated protein 1
NCBI Official Symbol
TRAP1  [Similar Products]
NCBI Official Synonym Symbols
HSP75; HSP 75; HSP90L; TRAP-1
  [Similar Products]
NCBI Protein Information
heat shock protein 75 kDa, mitochondrial; TNFR-associated protein 1; tumor necrosis factor type 1 receptor-associated protein
UniProt Protein Name
Heat shock protein 75 kDa, mitochondrial
UniProt Synonym Protein Names
TNFR-associated protein 1; Tumor necrosis factor type 1 receptor-associated protein; TRAP-1
UniProt Gene Name
TRAP1  [Similar Products]
UniProt Synonym Gene Names
HSP75; HSP 75; TRAP-1  [Similar Products]
UniProt Entry Name
TRAP1_HUMAN
NCBI Summary for TRAP1
This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
UniProt Comments for TRAP1
Function: Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, most likely through stabilization of mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA.3 PublicationsManual assertion based on experiment in:Ref.12
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with TRAP1 elisa kit
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