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TRIM35 elisa kit :: Human tripartite motif-containing 35 ELISA Kit

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Catalog # MBS9317166
Unit / Price
  48-Strip-Wells  /  $470 +1 FREE 8GB USB
  96-Strip-Wells  /  $680 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
TRIM35 elisa kit
Product Name

tripartite motif-containing 35 (TRIM35), ELISA Kit

Popular Item
Also Known As

Human Tripartite motif-containing protein 35, TRIM35 ELISA Kit

Product Synonym Names
Human Tripartite motif-containing protein 35 (TRIM35) ELISA kit; HLS5; KIAA1098; MAIR; MGC17233; ; tripartite motif-containing 35
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for Q9UPQ4
Species Reactivity
Specificity
No significant cross-reactivity or interference between Human TRIM35 and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces and Urine
Assay Type
Sandwich
Detection Range
0.25 ng/ml - 8 ng/ml.
Sensitivity
0.1 ng/ml.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TRIM35 concentrations in Human serum, plasma and other body fluids. Using Purified Human TRIM35 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TRIM35 and TRIM35 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TRIM35 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TRIM35 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTRIM35purchase
MBS9317166 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tripartite motif-containing 35 (TRIM35) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TRIM35. The ELISA analytical biochemical technique of the MBS9317166 kit is based on TRIM35 antibody-TRIM35 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TRIM35 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TRIM35. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TRIM35 elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TRIM35 concentrations in Human serum, plasma and other body fluids. Using Purified Human TRIM35 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TRIM35 and TRIM35 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TRIM35 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS9317166. Click to request current manual
NCBI/Uniprot data below describe general gene information for TRIM35. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
56,540 Da
NCBI Official Full Name
tripartite motif-containing protein 35
NCBI Official Synonym Full Names
tripartite motif containing 35
NCBI Official Symbol
TRIM35  [Similar Products]
NCBI Official Synonym Symbols
HLS5; MAIR
  [Similar Products]
NCBI Protein Information
tripartite motif-containing protein 35; tripartite motif-containing 35; hemopoietic lineage switch protein 5
UniProt Protein Name
Tripartite motif-containing protein 35
UniProt Synonym Protein Names
Hemopoietic lineage switch protein 5
UniProt Gene Name
TRIM35  [Similar Products]
UniProt Synonym Gene Names
HLS5; KIAA1098  [Similar Products]
UniProt Entry Name
TRI35_HUMAN
NCBI Summary for TRIM35
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The function of this protein has not been identified. [provided by RefSeq, Jul 2008]
UniProt Comments for TRIM35
TRIM35: May play a role as a tumor suppressor. Implicated in the cell death mechanism. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: zinc ion binding

Biological Process: negative regulation of cell proliferation; apoptosis; positive regulation of apoptosis; innate immune response; negative regulation of mitotic cell cycle
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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