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anti-Trimethyl-Histone H3 (Lys27) antibody :: Rabbit Trimethyl-Histone H3 (Lys27) Antibody

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Catalog # MBS462144
Unit / Price
  0.1 mg  /  $285 +1 FREE 8GB USB
Testing Data
Product Name

Trimethyl-Histone H3 (Lys27), Antibody

Full Product Name

Rabbit anti Trimethyl-Histone H3 (Lys 27)

Product Synonym Names
Rabbit anti-Trimethyl-Histone H3; Histone H3 trimethylated at Lys27 (H3-K27me3); H3K27[Me3].
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Host
Rabbit
Species Reactivity
Human, Rat, Mouse, Chicken, Bovine
Specificity
This antibody only recognizes ~ 17 kDa of Trimethylated Histone H3 at K27. It does not cross react to nonmethylated H3 or mono-, di-methylated Histone H3.
Purity/Purification
The Rabbit IgG is purified by Epitope-specific Affinity Chromatography.
Form/Format
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (lot specific)
Antigen Preparation
A synthetic peptide containing AR[Me3-K]SA in which Me3-K corresponds to trimethyl lysine 27 of human histone H3.
Positive Control
3T3 Nuclear Extract
Cellular Location
Nuclear
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Other Notes
Small volumes of anti-Trimethyl-Histone H3 (Lys27) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Trimethyl-Histone H3 (Lys27) antibody
Histones are basic nuclear proteins that together with DNA make up the nucleosome structure in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The methylation of specific residues in the histone tails is a central modification for regulatingepigenetic transitions in chromatin. Whereas methylation of histone H3 on lysine 4, 36, and 79 has been linked with gene activation, methylation of H3 on lysines 9, 27 and histone H4 on lysine 20 is associated with heterochromatin and some repressed genes with euchromatin. Modified lysine residues can exist in a mono-, di-, or tri methylated state, while the arginine residues can be mono- or di-methylated. Histone H3 Lys4 trimethylation (H3-K4me3) is a conserved mark of actively transcribed chromatin. This antibody is specific for histone H3 tri-methylated at K4. The sequence is found in all mammals and a wide range of species, including D. melanogaster, Arabidopsis, Chicken and Xenopus. The antibody will react with any of the above species where the trimethylation modification is present.
Applications Tested/Suitable for anti-Trimethyl-Histone H3 (Lys27) antibody
ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:100

Molecular Weight
17 kDa
Product References and Citations for anti-Trimethyl-Histone H3 (Lys27) antibody
Vakoc C et al. Histone H3 Lysine 9 Methylation and HP1gamma Are Associated with Transcription Elongation through Mammalian Chromatin. Molecular Cell (2003), Volume 19, Issue 3, Pages 381 - 391. David J Seward, et al. Demethylation of trimethylated histone H3 Lys4 in vivo by JARID1 JmjC proteins. Nature Structural & Molecular Biology 14, 240 - 242 (2007)

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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