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TSLP elisa kit :: Guinea Pig Thymic Stromal Lymphopoietin ELISA Kit

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Catalog # MBS723813
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Thymic Stromal Lymphopoietin (TSLP), ELISA Kit

Popular Item
Full Product Name

Guinea pig Thymic Stromal Lymphopoietin ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
607003
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of TSLP. No significant cross-reactivity or interference between TSLP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TSLP and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Detection Range
100-2500pg/mL
Sensitivity
1.0pg/mL
Intended Uses
This TSLP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig TSLP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TSLP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for TSLPpurchase
MBS723813 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Thymic Stromal Lymphopoietin (TSLP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TSLP. The ELISA analytical biochemical technique of the MBS723813 kit is based on TSLP antibody-TSLP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TSLP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TSLP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TSLP elisa kit
Principle of the assay: TSLP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TSLP. Standards or samples are then added to the microtiter plate wells and TSLP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TSLP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TSLP are added to each well to "sandwich" the TSLP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TSLP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TSLP concentration in each sample is interpolated from this standard curve.
Product Categories/Family for TSLP elisa kit

Typical Testing Data/Standard Curve (for reference only) of TSLP elisa kit
TSLP elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS723813. Click to request current manual
NCBI/Uniprot data below describe general gene information for TSLP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
18,141 Da
NCBI Official Full Name
thymic stromal lymphopoietin isoform 1
NCBI Official Synonym Full Names
thymic stromal lymphopoietin
NCBI Official Symbol
NCBI Protein Information
thymic stromal lymphopoietin
UniProt Protein Name
Thymic stromal lymphopoietin
UniProt Gene Name
UniProt Entry Name
TSLP_HUMAN
NCBI Summary for TSLP
This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]
UniProt Comments for TSLP
TSLP: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q22.1

Cellular Component: extracellular space

Molecular Function: cytokine activity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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