• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

TTK recombinant protein :: TTK Protein Kinase Recombinant Protein

Scan QR to view Datasheet
Catalog # MBS2029716 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $140 +1 FREE 8GB USB
  0.05 mg  /  $260 +1 FREE 8GB USB
  0.1 mg  /  $400 +1 FREE 8GB USB
  0.2 mg  /  $495 +1 FREE 8GB USB
  0.5 mg  /  $955 +1 FREE 8GB USB
  1 mg  /  $1,415 +1 FREE 8GB USB
Product Name

TTK Protein Kinase, Recombinant Protein

Popular Item
Also Known As

Recombinant TTK Protein Kinase (TTK)

Matching Pairs
Matching Pairs
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
857
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHH H SSGLVPRGSH MASMTGGQQM GRGSEF- GSGGSSKVF QVLNEKKQIY AIKYVNLEEA DNQTLDSYRN EIAYLNKLQQ HSDKIIRLYD YEITDQYIYM VMECGNIDLN SWLKKKKSID PWERKSYWKN MLEAVHTIHQ HGIVHSDLKP ANFLIVDGML KLIDFGIANQ MQPDTTSVVK DSQVGTVNYM PPEAIKDMSS SRENGKSKSK ISPKSDVWSL GCILYYMTYG KTPFQQIINQ ISKLHAIIDP NHEIEFPDIP EKDLQDVLKC CLKRDPKQRI SIPELL
OMIM
604092
Host
Host: E Coli
Source: Prokaryotic expression
Purity/Purification
>95%
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted Molecular Mass
33.3kDa
Accurate Molecular Mass (KD)
33kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
N-terminal His-Tag, T7 tag
Organism Species
Homo sapiens (Human)
Fragment
Gly532~Leu786
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Other Notes
Small volumes of TTK recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for TTK recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Sequence Information of TTK recombinant protein
TTK recombinant protein Sequence Information image
NCBI/Uniprot data below describe general gene information for TTK. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
96,944 Da
NCBI Official Full Name
TTK protein kinase
NCBI Official Synonym Full Names
TTK protein kinase
NCBI Official Symbol
NCBI Official Synonym Symbols
ESK; PYT; CT96; MPH1; MPS1; MPS1L1
  [Similar Products]
NCBI Protein Information
dual specificity protein kinase TTK
UniProt Protein Name
Dual specificity protein kinase TTK
UniProt Synonym Protein Names
Phosphotyrosine picked threonine-protein kinase; PYT
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
MPS1; MPS1L1; PYT  [Similar Products]
UniProt Entry Name
TTK_HUMAN
NCBI Summary for TTK
This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2009]
UniProt Comments for TTK
TTK: a dual-specificity protein kinase orthologous to the yeast monopolar spindle (Mps1) kinase that is required for spindle pole duplication and the spindle checkpoint but not for centrosome duplication. Localized to centrosomes, kinetochores and nuclear pores. Its enzymatic activity is required for the recruitment of Mad1, Mad2, Bub1 and Bub3 to the kinetochore. Mps1 mRNA levels change little throughout the cell cycle, but its protein expression varies during the cell cycle. Overexpression arrests cells in metaphase by activating the checkpoint.

Protein type: Kinase, protein; Cancer Testis Antigen (CTA); Protein kinase, dual-specificity (non-receptor); EC 2.7.12.1; Protein kinase, Other; Other group; TTK family

Chromosomal Location of Human Ortholog: 6q14.1

Cellular Component: membrane; spindle

Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; ATP binding

Biological Process: peptidyl-tyrosine phosphorylation; mitotic spindle organization and biogenesis; positive regulation of cell proliferation; mitotic cell cycle spindle assembly checkpoint; spindle organization and biogenesis; chromosome separation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000