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TUFT1 elisa kit :: Human tuftelin 1 ELISA Kit

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Catalog # MBS939358
Unit / Price DISCONTINUED
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Typical Testing Data/Standard Curve (for reference only)
Product Name

tuftelin 1 (TUFT1), ELISA Kit

Popular Item
Also Known As

Human Tuftelin, TUFT1 ELISA Kit

Product Synonym Names
Human Tuftelin (TUFT1) ELISA kit; tuftelin 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS939358 Testing Data
Sequence Length
390
OMIM
600087
3D Structure
ModBase 3D Structure for Q9NNX1
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human TUFT1. No significant cross-reactivity or interference between Human TUFT1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
0.156 ng/ml-10 ng/ml
Sensitivity
0.039 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Biosynthesis/Metabolism
Protein Biological Process 3
Biomineralization
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TUFT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTUFT1purchase
MBS939358 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tuftelin 1 (TUFT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TUFT1. The ELISA analytical biochemical technique of the MBS939358 kit is based on TUFT1 antibody-TUFT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TUFT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TUFT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TUFT1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TUFT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TUFT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TUFT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TUFT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of TUFT1 elisa kit
TUFT1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS939358. Click to request current manual
NCBI/Uniprot data below describe general gene information for TUFT1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
44,264 Da
NCBI Official Full Name
tuftelin isoform 2
NCBI Official Synonym Full Names
tuftelin 1
NCBI Official Symbol
TUFT1  [Similar Products]
NCBI Protein Information
tuftelin
UniProt Protein Name
Tuftelin
Protein Family
UniProt Gene Name
TUFT1  [Similar Products]
UniProt Entry Name
TUFT1_HUMAN
NCBI Summary for TUFT1
Tuftelin is an acidic protein that is thought to play a role in dental enamel mineralization and is implicated in caries susceptibility. It is also thought to be involved with adaptation to hypoxia, mesenchymal stem cell function, and neurotrophin nerve growth factor mediated neuronal differentiation. [provided by RefSeq, Aug 2014]
UniProt Comments for TUFT1
TUFT1: Involved in the mineralization and structural organization of enamel. Belongs to the tuftelin family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoplasm; extracellular region

Molecular Function: structural constituent of tooth enamel

Biological Process: odontogenesis; bone mineralization
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Diseases associated with TUFT1 elisa kit
Organs/Tissues associated with TUFT1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Mouth Antibodies  >2 publications with TUFT1 and Mouth
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