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TYMS recombinant protein :: Thymidylate synthase Recombinant Protein

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Catalog # MBS203615
Unit / Price
  0.1 mg  /  $285 +1 FREE 8GB USB
  0.5 mg  /  $640 +1 FREE 8GB USB
SDS-Page
Product Name

Thymidylate synthase (TYMS), Recombinant Protein

Popular Item
Full Product Name

Thymidylate synthase, 1-313aa, Human, His tag, E Coli

Product Synonym Names
HST422; TMS; TS; d TMP synthase; EC 2.1.1.45; MGC88736; Thymidylate synthase; Thymidylate synthetase; Tsase; TYMS; TYMS protein; Tyms thymidylate synthetase
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MGSSHHHHHH SSGLVPRGSH MPVAGSELPR RPLPPAAQER DAEPRPPHGE LQYLGQIQHI LRCGVRKDDR TGTGTLSVFG MQARYSLRDE FPLLTTKRVF WKGVLEELLW FIKGSTNAKE LSSKGVKIWD ANGSRDFLDS LGFSTREEGD LGPVYGFQWR HFGAEYRDME SDYSGQGVDQ LQRVIDTIKT NPDDRRIIMC AWNPRDLPLM ALPPCHALCQ FYVVNSELSC QLYQRSGDMG LGVPFNIASY ALLTYMIAHI TGLKPGDFIH TLGDAHIYLN HIEPLKIQLQ REPRPFPKLR ILRKVEKIDD FKAEDFQIEG YNPHPTIKME MAV
OMIM
X02308 mRNA
3D Structure
ModBase 3D Structure for P04818
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1 M NaCl.
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of TYMS recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TYMS recombinant protein
Thymidylate synthase is an intracellular enzyme critical for de novo synthesis of DNA. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. In cancer, expression of this protein is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-Fu). Resistance or lack of response to 5-Fu is attributed to the elevation of thymidylate synthase activity. Recombinant human Thymidylate synthase protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for TYMS recombinant protein
Applications Tested/Suitable for TYMS recombinant protein
SDS-PAGE

NCBI/Uniprot data below describe general gene information for TYMS. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
37.8 kDa (333aa), confirmed by MALDI-TOF. [Similar Products]
NCBI Official Full Name
thymidylate synthase
NCBI Official Synonym Full Names
thymidylate synthetase
NCBI Official Symbol
NCBI Official Synonym Symbols
TS; TMS; HST422
  [Similar Products]
NCBI Protein Information
thymidylate synthase
UniProt Protein Name
Thymidylate synthase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
TS; TS; TSase  [Similar Products]
NCBI Summary for TYMS
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy. [provided by RefSeq, Aug 2017]
UniProt Comments for TYMS
Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
Product References and Citations for TYMS recombinant protein
Chiu TH., et al. (2009) Anticancer Res. 29 (11):4503-11. ; Pena MM., et al. (2009) J Biol Chem. 284 (46):31597-607.;

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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