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UBE2M recombinant protein :: Ubiquitin Conjugating Enzyme E2M Recombinant Protein

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Catalog # MBS142486
Unit / Price
  0.005 mg  /  $140 +1 FREE 8GB USB
  0.02 mg  /  $205 +1 FREE 8GB USB
  1 mg  /  $2,665 +3 FREE 8GB USB
UBE2M recombinant protein
Product Name

Ubiquitin Conjugating Enzyme E2M (UBE2M), Recombinant Protein

Also Known As

Recombinant Human Ubiquitin Conjugating Enzyme E2M

Product Synonym Names
UBE2M Human; Ubiquitin Conjugating Enzyme E2M Human Recombinant; NEDD8-conjugating enzyme Ubc12; Ubiquitin-conjugating enzyme E2 M; NEDD8 protein ligase; NEDD8 carrier protein; UBC12; hUbc12; UBC-RS2
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
183
Sequence
MGSSHHH HHH SSGLVPRGSH MGSHMI KLFS LKQQKKEEES AGGTKGSSKK ASAAQLRIQK DINELNLPKT CDISFSDPDD LLNFKLVICP DEGFYKSGKF VFSFKVGQGY PHDPPKVKCE TMVYHPNIDL EGNVCLNILR EDWKPVLTIN SIIYGLQYLF LEPNPEDPLN KEAAEVLQNN RRLFEQNVQR SMRGGYIGST YFERCLK
OMIM
603173
3D Structure
ModBase 3D Structure for P61081
Host
E Coli
Purity/Purification
Greater than 95% as determined by SDS-PAGE.
Form/Format
The UBE2M solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 1mM DTT and 20% glycerol.
Sterile filtered colorless solution.
Other Notes
Small volumes of UBE2M recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
UBE2M recombinant protein
Description: UBE2M Human Recombinant produced E Coli is a single polypeptide chain containing 207 amino acids (1-183) and having a molecular mass of 23.5kDa.UBE2M is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: UbcH12 is functional in in vitro NEDDylation reactions. It has been shown to form a thioester linkage with NEDD8 in the presence of the NEDD8 activating enzyme complex Uba3/APP-BP1. APP-BP1 binds to the amyloid precursor protein (APP) carboxy terminal domain and is important in conjunction with Uba3 and UbcH12 in driving cells through the S to M checkpoint. It was demonstrated to be the E2 responsible for the NEDDylation of the Cul-1 component of the SCF (?-TRCP) complex which is important as the E3-ligase in the ubiquitinylation of I?B?. NEDDylation of Cul-1 is essential for conjugation and processing of NF-?B p105 by SCF (?-TRCP) following phosphorylation of the complex. A dominant negative form of UbcH12, previously demonstrated to sequester NEDD8 and inhibit its conjugation, inhibits both conjugation and processing of p105, which is alleviated by wild-type UbcH12.
NCBI/Uniprot data below describe general gene information for UBE2M. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
20,900 Da
NCBI Official Full Name
NEDD8-conjugating enzyme Ubc12
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2M
NCBI Official Symbol
UBE2M  [Similar Products]
NCBI Official Synonym Symbols
UBC12; hUbc12; UBC-RS2
  [Similar Products]
NCBI Protein Information
NEDD8-conjugating enzyme Ubc12; NEDD8 carrier protein; NEDD8 protein ligase; ubiquitin carrier protein M; ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast); ubiquitin-protein ligase M; yeast UBC12 homolog
UniProt Protein Name
NEDD8-conjugating enzyme Ubc12
UniProt Synonym Protein Names
NEDD8 carrier protein; NEDD8 protein ligase; Ubiquitin-conjugating enzyme E2 M
UniProt Gene Name
UBE2M  [Similar Products]
UniProt Synonym Gene Names
UBC12  [Similar Products]
UniProt Entry Name
UBC12_HUMAN
NCBI Summary for UBE2M
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008]
UniProt Comments for UBE2M
UBE2M: Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation. Interacts with UBA3, DCUN1D1 and RBX1. Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.

Protein type: Ubiquitin ligase; Ligase; Adaptor/scaffold; Ubiquitin conjugating system; EC 6.3.2.19; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 19q13.43

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ribosomal S6-glutamic acid ligase activity; ATP binding; NEDD8 ligase activity

Biological Process: protein neddylation; positive regulation of neuron apoptosis; transforming growth factor beta receptor signaling pathway; protein modification process; protein ubiquitination
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with UBE2M recombinant protein
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