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UCN elisa kit :: Mouse urocortin ELISA Kit

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Catalog # MBS941797
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $435 +1 FREE 8GB USB
  96-Strip-Wells  /  $625 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,905 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

urocortin (UCN), ELISA Kit

Popular Item
Full Product Name

Mouse Urocortin, UCN ELISA Kit

Product Synonym Names
Mouse Urocortin (UCN) ELISA kit; MGC129974; MGC129975; UI; UROC; urocortin; preproprotein; urocortin
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS941797 Testing Data
3D Structure
ModBase 3D Structure for P81615
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse urocortin. No significant cross-reactivity or interference between mouse urocortin and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell culture supernates
Assay Type
Sandwich
Detection Range
12.5 pg/ml-800 pg/ml.
Sensitivity
The minimum detectable dose of mouse urocortin is typically less than 3.12 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of UCN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forUCNpurchase
MBS941797 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the urocortin (UCN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing UCN. The ELISA analytical biochemical technique of the MBS941797 kit is based on UCN antibody-UCN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect UCN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, UCN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
UCN elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for urocortin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any urocortin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for urocortin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of urocortin bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of UCN elisa kit
UCN elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS941797. Click to request current manual
NCBI/Uniprot data below describe general gene information for UCN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
13,558 Da
NCBI Official Full Name
urocortin
NCBI Official Synonym Full Names
urocortin
NCBI Official Symbol
NCBI Official Synonym Symbols
Ucn1; Mpv17
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NCBI Protein Information
urocortin
UniProt Protein Name
Urocortin
Protein Family
UniProt Gene Name
UniProt Entry Name
UCN1_MOUSE
UniProt Comments for UCN
UCN: Acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH). Binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta. Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: cell soma; axon; dendrite; extracellular region; perikaryon; nerve terminal

Molecular Function: corticotropin-releasing hormone receptor 1 binding; histone deacetylase inhibitor activity; G-protein-coupled receptor binding; hormone activity; corticotropin-releasing hormone receptor 2 binding

Biological Process: regulation of synaptic transmission, glutamatergic; positive regulation of translation; negative regulation of hormone secretion; positive regulation of collagen biosynthetic process; drinking behavior; negative regulation of cell size; pancreatic juice secretion; positive regulation of vasodilation; negative regulation of appetite; positive regulation of adrenocorticotropic hormone secretion; sensory perception of sound; learning and/or memory; neuropeptide signaling pathway; positive regulation of cAMP biosynthetic process; feeding behavior; negative regulation of blood pressure; neurite development; associative learning; negative regulation of histone deacetylation; activation of protein kinase A; positive regulation of interleukin-6 production; social behavior; positive regulation of cell growth; positive regulation of peptidyl-serine phosphorylation; positive regulation of vascular permeability; negative regulation of catalytic activity; aerobic respiration; response to oxidative stress; positive regulation of protein amino acid phosphorylation; positive regulation of DNA replication; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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