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UCP1 elisa kit :: Horse Uncoupling Protein 1, Mitochondrial ELISA Kit

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Catalog # MBS066225
Unit / Price
  48-Strip-Wells  /  $470 +1 FREE 8GB USB
  96-Strip-Wells  /  $680 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
UCP1 elisa kit
Product Name

Uncoupling Protein 1, Mitochondrial (UCP1), ELISA Kit

Popular Item
Full Product Name

Horse Uncoupling Protein 1, Mitochondrial ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for O81845
Species Reactivity
Specificity
No significant cross-reactivity or interference between Horse UCP1 and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
Assay Type
Sandwich
Detection Range
62.5 pg/ml - 2000 pg/ml.
Sensitivity
10 pg/ml.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated UCP1 concentrations in Horse serum, plasma and other body fluids. Using Purified Horse UCP1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add UCP1 and UCP1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of UCP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of UCP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forUCP1purchase
MBS066225 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Uncoupling Protein 1, Mitochondrial (UCP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing UCP1. The ELISA analytical biochemical technique of the MBS066225 kit is based on UCP1 antibody-UCP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect UCP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, UCP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
UCP1 elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated UCP1 concentrations in Horse serum, plasma and other body fluids. Using Purified Horse UCP1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add UCP1 and UCP1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of UCP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS066225. Click to request current manual
NCBI/Uniprot data below describe general gene information for UCP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
32,662 Da
NCBI Official Full Name
uncoupling family protein PUMP2
NCBI Official Symbol
PUMP1  [Similar Products]
NCBI Official Synonym Symbols
ARABIDOPSIS THALIANA PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 1; ARABIDOPSIS THALIANA UNCOUPLING PROTEIN 1; ATPUMP1; ATUCP1; plant uncoupling mitochondrial protein 1; PUMP1; UCP; UCP1; UNCOUPLING PROTEIN 1
  [Similar Products]
NCBI Protein Information
uncoupling family protein PUMP2
UniProt Protein Name
Mitochondrial uncoupling protein 1
UniProt Gene Name
PUMP1  [Similar Products]
UniProt Synonym Gene Names
UCP1; AtPUMP1  [Similar Products]
UniProt Entry Name
PUMP1_ARATH
NCBI Summary for UCP1
Member of Uncoupling protein PUMP2 family. Encodes a mitochondrial uncoupling protein AtUCP1 involved in maintain the redox poise of the mitochondrial electron transport chain to facilitate photosynthetic metabolism. Disruption of UCP1 results in a photosynthetic phenotype. Specifically there is a restriction in photorespiration with a decrease in the rate of oxidation of photorespiratory glycine in the mitochondrion. This change leads to an associated reduced photosynthetic carbon assimilation rate.
UniProt Comments for UCP1
Function: PUMPS are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. This leads to a decrease in the efficiency of oxidative phosphorylation and an increase in heat production. Is involved in protecting plant cells against oxidative stress damage and maintaining the redox balance of the mitochondrial electron transport chain to facilitate photosynthetic metabolism. May play a regulatory role during photorespiration. Ref.8 Ref.9 Ref.12 Ref.13 Ref.15 Ref.16

Subcellular location: Mitochondrion inner membrane; Multi-pass membrane protein

By similarity.

Tissue specificity: Widely expressed. Ref.1 Ref.12

Induction: By cold stress, high light and chloramphenicol. Ref.1 Ref.10 Ref.14

Disruption phenotype: Slight reduction of shoot mass. Ref.12

Miscellaneous: Over-expression of Arabidopsis UCP1 in tobacco plants increases tolerance to oxidative stress caused by exogenous hydrogen peroxide, or by drought and salt (Ref.9).

Sequence similarities: Belongs to the mitochondrial carrier (TC 2.A.29) family. [View classification]Contains 3 Solcar repeats.

Sequence caution: The sequence BAD95028.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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