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UGT1A1 elisa kit :: Rat UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1) ELISA Kit

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Catalog # MBS2020273 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  24-Strip-Wells  /  $285 +1 FREE 8GB USB
  48-Strip-Wells  /  $530 +1 FREE 8GB USB
  96-Strip-Wells  /  $715 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,910 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,280 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1), ELISA Kit

Also Known As

UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1) ELISA Kit

Product Synonym Names
UGT1; GNT1; UGT1A; UDPGT; HUG-BR1; UGT1-1; Bilirubin-specific UDPGT isozyme 1; UDP-glucuronosyltransferase 1-1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2020273 MSDS
Sequence Length
533
OMIM
143500
3D Structure
ModBase 3D Structure for P22309
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1).
No significant cross-reactivity or interference between UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates and Other Biological Fluids
Detection Range
1.56-100ng/mL
Sensitivity
< 0.61ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of UGT1A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forUGT1A1purchase
MBS2020273 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing UGT1A1. The ELISA analytical biochemical technique of the MBS2020273 kit is based on UGT1A1 antibody-UGT1A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect UGT1A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, UGT1A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
UGT1A1 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for UGT1A1 elisa kit

Typical Testing Data/Standard Curve (for reference only) of UGT1A1 elisa kit
UGT1A1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2020273. Click to request current manual
NCBI/Uniprot data below describe general gene information for UGT1A1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
49,368 Da
NCBI Official Full Name
UDP glucuronosyltransferase 1 family, polypeptide A1
NCBI Official Synonym Full Names
UDP glucuronosyltransferase 1 family, polypeptide A1
NCBI Official Symbol
UGT1A1  [Similar Products]
NCBI Official Synonym Symbols
GNT1; UGT1; UDPGT; UGT1A; HUG-BR1; BILIQTL1; UDPGT 1-1
  [Similar Products]
NCBI Protein Information
UDP-glucuronosyltransferase 1-1
UniProt Protein Name
UDP-glucuronosyltransferase 1-1
UniProt Synonym Protein Names
Bilirubin-specific UDPGT isozyme 1; hUG-BR1; UDP-glucuronosyltransferase 1-A; UGT-1A; UGT1A; UDP-glucuronosyltransferase 1A1
UniProt Gene Name
UGT1A1  [Similar Products]
UniProt Synonym Gene Names
GNT1; UGT1; UDPGT 1-1; UGT1*1; UGT1-01; UGT1.1; hUG-BR1; UGT-1A; UGT1A  [Similar Products]
UniProt Entry Name
UD11_HUMAN
NCBI Summary for UGT1A1
This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids. Mutations in this gene result in Crigler-Najjar syndromes types I and II and in Gilbert syndrome. [provided by RefSeq, Jul 2008]
UniProt Comments for UGT1A1
UGT1A1: UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform glucuronidates bilirubin IX- alpha to form both the IX-alpha-C8 and IX-alpha-C12 monoconjugates and diconjugate. Is also able to catalyze the glucuronidation of 17beta-estradiol, 17alpha-ethinylestradiol, 1-hydroxypyrene, 4- methylumbelliferone, 1-naphthol, paranitrophenol, scopoletin, and umbelliferone. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Expressed in liver. Not expressed in skin or kidney. Belongs to the UDP-glycosyltransferase family. 1 isoforms of the human protein are produced by alternative splicing.

Protein type: Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Xenobiotic Metabolism - metabolism by cytochrome P450; Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - ascorbate and aldarate; Xenobiotic Metabolism - drug metabolism - other enzymes; EC 2.4.1.17; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Transferase; Cofactor and Vitamin Metabolism - retinol; Carbohydrate Metabolism - pentose and glucuronate interconversions; Membrane protein, integral; Lipid Metabolism - androgen and estrogen

Chromosomal Location of Human Ortholog: 2q37

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to plasma membrane

Molecular Function: enzyme inhibitor activity; protein homodimerization activity; enzyme binding; retinoic acid binding; glucuronosyltransferase activity; protein heterodimerization activity; steroid binding

Biological Process: steroid metabolic process; response to drug; estrogen metabolic process; negative regulation of steroid metabolic process; organ regeneration; response to lipopolysaccharide; negative regulation of fatty acid metabolic process; liver development; cellular response to hormone stimulus; response to starvation; negative regulation of transferase activity; flavonoid biosynthetic process; bilirubin conjugation; heme catabolic process; xenobiotic metabolic process; porphyrin metabolic process; digestion; negative regulation of catalytic activity; flavone metabolic process; acute-phase response; retinoic acid metabolic process; heterocycle metabolic process; drug metabolic process; response to nutrient

Disease: Gilbert Syndrome; Crigler-najjar Syndrome, Type I; Bilirubin, Serum Level Of, Quantitative Trait Locus 1; Hyperbilirubinemia, Transient Familial Neonatal; Crigler-najjar Syndrome, Type Ii
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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