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ULBP2 elisa kit :: Canine UL16 Binding protein 2 ELISA Kit

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Catalog # MBS734130
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
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Typical Testing Data/Standard Curve (for reference only)
Product Name

UL16 Binding protein 2 (ULBP2), ELISA Kit

Popular Item
Full Product Name

Canine UL16 Binding protein 2 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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OMIM
605698
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of ULBP2. No significant cross-reactivity or interference between ULBP2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ULBP2 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/mL
Intended Uses
This ULBP2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine ULBP2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ULBP2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ULBP2purchase
MBS734130 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the UL16 Binding protein 2 (ULBP2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ULBP2. The ELISA analytical biochemical technique of the MBS734130 kit is based on ULBP2 antibody-ULBP2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ULBP2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ULBP2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ULBP2 elisa kit
Principle of the assay: ULBP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ULBP2 antibody and an ULBP2-HRP conjugate. The assay sample and buffer are incubated together with ULBP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ULBP2 concentration since ULBP2 from samples and ULBP2-HRP conjugate compete for the anti-ULBP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ULBP2 from the sample, fewer sites are left to bind ULBP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ULBP2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ULBP2 elisa kit

Typical Testing Data/Standard Curve (for reference only) of ULBP2 elisa kit
ULBP2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS734130. Click to request current manual
NCBI/Uniprot data below describe general gene information for ULBP2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
27,368 Da
NCBI Official Full Name
UL16 binding protein 2
NCBI Official Synonym Full Names
UL16 binding protein 2
NCBI Official Symbol
ULBP2  [Similar Products]
NCBI Official Synonym Symbols
N2DL2; RAET1H
  [Similar Products]
NCBI Protein Information
NKG2D ligand 2; N2DL-2; NKG2DL2; ALCAN-alpha; UL16-binding protein 2; retinoic acid early transcript 1H; retinoic acid early transcript 1 H
UniProt Protein Name
NKG2D ligand 2
UniProt Synonym Protein Names
ALCAN-alpha; Retinoic acid early transcript 1H; UL16-binding protein 2
Protein Family
UniProt Gene Name
ULBP2  [Similar Products]
UniProt Synonym Gene Names
N2DL2; RAET1H; UNQ463/PRO791; N2DL-2; NKG2DL2  [Similar Products]
UniProt Entry Name
N2DL2_HUMAN
NCBI Summary for ULBP2
This gene encodes a major histocompatibility complex (MHC) class I-related molecule that binds to the NKG2D receptor on natural killer (NK) cells to trigger release of multiple cytokines and chemokines that in turn contribute to the recruitment and activation of NK cells. The encoded protein undergoes further processing to generate the mature protein that is either anchored to membrane via a glycosylphosphatidylinositol moiety, or secreted. Many malignant cells secrete the encoded protein to evade immunosurveillance by NK cells. This gene is located in a cluster of multiple MHC class I-related genes on chromosome 6. [provided by RefSeq, Jul 2015]
UniProt Comments for ULBP2
ULBP2: Ligand for the NKG2D receptor, together with at least ULBP1 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP2 to be retained in the ER and cis- Golgi apparatus so that it does not reach the cell surface. Belongs to the MHC class I family.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 6q25

Cellular Component: extracellular space; anchored to plasma membrane; cell surface

Molecular Function: peptide antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; natural killer cell mediated cytotoxicity; natural killer cell activation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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