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VCAM-1/CD106 elisa kit :: Rat vascular cell adhesion protein 1 (VCAM-1/CD106) ELISA Kit

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Catalog # MBS703064
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $590 +1 FREE 8GB USB
  96-Strip-Wells  /  $855 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,110 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,890 +7 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

vascular cell adhesion protein 1 (VCAM-1/CD106), ELISA Kit

Popular Item
Also Known As

Rat vascular cell adhesion protein 1, VCAM-1/CD106 ELISA Kit

Product Synonym Names
Rat vascular cell adhesion protein 1 (VCAM-1/CD106) ELISA kit; CD106; DKFZp779G2333; INCAM-100; MGC99561; CD106 antigen; vascular cell adhesion protein 1 (VCAM-1/CD106)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS703064 Testing Data
Sequence Length
739
3D Structure
ModBase 3D Structure for P29534
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of rat VCAM-1. No significant cross-reactivity or interference between rat VCAM-1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Detection Range
15.6 ng/ml -1000 ng/mL
Sensitivity
<3.9 ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of VCAM-1/CD106 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forVCAM-1/CD106purchase
MBS703064 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the vascular cell adhesion protein 1 (VCAM-1/CD106) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing VCAM-1/CD106. The ELISA analytical biochemical technique of the MBS703064 kit is based on VCAM-1/CD106 antibody-VCAM-1/CD106 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VCAM-1/CD106 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VCAM-1/CD106. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
VCAM-1/CD106 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VCAM-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VCAM-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VCAM-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VCAM-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of VCAM-1/CD106 elisa kit
VCAM-1/CD106 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS703064. Click to request current manual
NCBI/Uniprot data below describe general gene information for VCAM-1/CD106. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
81,246 Da
NCBI Official Full Name
vascular cell adhesion protein 1
NCBI Official Synonym Full Names
vascular cell adhesion molecule 1
NCBI Official Symbol
Vcam1  [Similar Products]
NCBI Official Synonym Symbols
VCAM1B
  [Similar Products]
NCBI Protein Information
vascular cell adhesion protein 1; VCAM-1; V-CAM 1
UniProt Protein Name
Vascular cell adhesion protein 1
UniProt Synonym Protein Names
CD_antigen: CD106
UniProt Gene Name
Vcam1  [Similar Products]
UniProt Synonym Gene Names
Vcam-1; V-CAM 1; VCAM-1  [Similar Products]
UniProt Entry Name
VCAM1_RAT
NCBI Summary for VCAM-1/CD106
binds integrin very late antigen (VLA4); plays a role in cell adhesion [RGD, Feb 2006]
UniProt Comments for VCAM-1/CD106
Function: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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