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VCAM-1/CD106 elisa kit :: Rat VCAM-1 ELISA Kit

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Catalog # MBS2502676
Unit / Price
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  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

VCAM-1 (VCAM-1/CD106), ELISA Kit

Popular Item
Full Product Name

Rat VCAM-1 (Vascuolar Cell Adhesion Molecule 1) ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2502676 COA
3D Structure
ModBase 3D Structure for P29534
Species Reactivity
Specificity
This kit recognizes natural and some recombinant Rat VCAM-1. No significant crossreactivity or interference between Rat VCAM-1 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
12.5-800pg/mL
Sensitivity
Min: 7.5pg/mL; Max: 800pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of VCAM-1/CD106 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forVCAM-1/CD106purchase
MBS2502676 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the VCAM-1 (VCAM-1/CD106) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing VCAM-1/CD106. The ELISA analytical biochemical technique of the MBS2502676 kit is based on VCAM-1/CD106 antibody-VCAM-1/CD106 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VCAM-1/CD106 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VCAM-1/CD106. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
VCAM-1/CD106 elisa kit
Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Rat VCAM-1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Rat VCAM-1. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Rat VCAM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Rat VCAM-1, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat VCAM-1. The concentration of Rat VCAM-1 in samples can be calculated by comparing the OD of the samples with the standard curve.

Typical Testing Data/Standard Curve (for reference only) of VCAM-1/CD106 elisa kit
VCAM-1/CD106 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2502676. Click to request current manual
NCBI/Uniprot data below describe general gene information for VCAM-1/CD106. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
81,246 Da
NCBI Official Full Name
vascular cell adhesion protein 1
NCBI Official Synonym Full Names
vascular cell adhesion molecule 1
NCBI Official Symbol
Vcam1  [Similar Products]
NCBI Official Synonym Symbols
VCAM1B
  [Similar Products]
NCBI Protein Information
vascular cell adhesion protein 1; V-CAM 1; VCAM-1
UniProt Protein Name
Vascular cell adhesion protein 1
UniProt Synonym Protein Names
CD_antigen: CD106
UniProt Gene Name
Vcam1  [Similar Products]
UniProt Synonym Gene Names
Vcam-1; V-CAM 1; VCAM-1  [Similar Products]
UniProt Entry Name
VCAM1_RAT
NCBI Summary for VCAM-1/CD106
binds integrin very late antigen (VLA4); plays a role in cell adhesion [RGD, Feb 2006]
UniProt Comments for VCAM-1/CD106
VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Cellular Component: Golgi apparatus; extracellular space; microvillus; cell surface; endoplasmic reticulum; apical part of cell; early endosome; integral to membrane; podosome; sarcolemma; filopodium; external side of plasma membrane

Molecular Function: integrin binding; amine oxidase activity; cell adhesion molecule binding

Biological Process: response to nicotine; chronic inflammatory response; viral reproduction; heart development; cell-matrix adhesion; response to lipopolysaccharide; heterophilic cell adhesion; leukocyte adhesion; response to ethanol; response to zinc ion; membrane to membrane docking; response to hypoxia; positive regulation of T cell proliferation; response to ionizing radiation; amine metabolic process; cell adhesion; leukocyte tethering or rolling; acute inflammatory response; response to nutrient; aging
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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