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VEGF-D elisa kit :: Rat VEGF-D ELISA Kit

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Catalog # MBS2508749 (SPECIAL PROMOTIONAL PRICING for a limited time)
Unit / Price
  48-Strip-Wells  /  $310 +1 FREE 8GB USB
  96-Strip-Wells  /  $355 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,435 +1 FREE 8GB USB
  10x96-Strip-Wells  /  $2,805 +3 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

VEGF-D, ELISA Kit

Popular Item
Also Known As

Rat VEGF-D (Vascular Endothelial Cell Growth Factor D) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2508749 COA
Sequence Length
354
OMIM
300091
3D Structure
ModBase 3D Structure for O43915
Species Reactivity
Specificity
This kit recognizes natural and recombinant Rat VEGF-D. No significant cross-reactivity or interference between Rat VEGF-D and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
15.625-1000pg/mL
Sensitivity
Min: 9.375pg/mL; Max: 1000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of VEGF-D elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forVEGF-Dpurchase
MBS2508749 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the VEGF-D, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing VEGF-D. The ELISA analytical biochemical technique of the MBS2508749 kit is based on VEGF-D antibody-VEGF-D antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VEGF-D antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VEGF-D. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
VEGF-D elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat VEGF-D concentrations in serum, plasma and other biological fluids.

Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to VEGF-D. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for VEGF-D and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain VEGF-D, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of VEGF-D. You can calculate the concentration of VEGF-D in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of VEGF-D elisa kit
VEGF-D elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2508749. Click to request current manual
NCBI/Uniprot data below describe general gene information for VEGF-D. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40,444 Da
NCBI Official Full Name
vascular endothelial growth factor D preproprotein
NCBI Official Synonym Full Names
c-fos induced growth factor (vascular endothelial growth factor D)
NCBI Official Symbol
NCBI Official Synonym Symbols
VEGFD; VEGF-D
  [Similar Products]
NCBI Protein Information
vascular endothelial growth factor D
UniProt Protein Name
Vascular endothelial growth factor D
UniProt Synonym Protein Names
c-Fos-induced growth factor; FIGF
UniProt Gene Name
UniProt Synonym Gene Names
VEGFD; VEGF-D; FIGF  [Similar Products]
UniProt Entry Name
VEGFD_HUMAN
NCBI Summary for VEGF-D
The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor C. Read-through transcription has been observed between this locus and the upstream PIR (GeneID 8544) locus. [provided by RefSeq, Feb 2011]
UniProt Comments for VEGF-D
VEGFD: Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. Belongs to the PDGF/VEGF growth factor family.

Protein type: Secreted, signal peptide; Cytokine; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: Xp22.31

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: protein homodimerization activity; growth factor activity; platelet-derived growth factor receptor binding; vascular endothelial growth factor receptor 3 binding; vascular endothelial growth factor receptor binding; chemoattractant activity

Biological Process: platelet activation; cell proliferation; platelet degranulation; positive chemotaxis; positive regulation of cell division; positive regulation of cell proliferation; angiogenesis; vascular endothelial growth factor receptor signaling pathway; blood coagulation; cell differentiation; induction of positive chemotaxis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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