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VIM elisa kit :: Rat vimentin ELISA Kit

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Catalog # MBS702882
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Typical Testing Data/Standard Curve (for reference only)
Product Name

vimentin, ELISA Kit

Popular Item
Full Product Name

Rat vimentin, VIM ELISA Kit

Product Synonym Names
Rat vimentin (VIM) ELISA Kit; RP11-124N14.1; FLJ36605; ; vimentin
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS702882 Testing Data
3D Structure
ModBase 3D Structure for P31000
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of rat VIM. No significant cross-reactivity or interference between rat VIM and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
23.5 ng/ml-1500 ng/ml.
Sensitivity
The minimum detectable dose of rat VIM is typically less than 5.8 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of VIM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forVIMpurchase
MBS702882 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the vimentin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing VIM. The ELISA analytical biochemical technique of the MBS702882 kit is based on VIM antibody-VIM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VIM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VIM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
VIM elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VIM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VIM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VIM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VIM bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of VIM elisa kit
VIM elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS702882. Click to request current manual
NCBI/Uniprot data below describe general gene information for VIM. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
53,733 Da
NCBI Official Full Name
vimentin
NCBI Official Synonym Full Names
vimentin
NCBI Official Symbol
NCBI Protein Information
vimentin
UniProt Protein Name
Vimentin
Protein Family
UniProt Gene Name
UniProt Entry Name
VIME_RAT
NCBI Summary for VIM
class-III intermediate filament of the cytoskeleton; along with desmin (the subunit specifically expressed in muscle), vimentin is specific for mesenchymal tissue [RGD, Feb 2006]
UniProt Comments for VIM
Function: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.1 PublicationManual assertion based on experiment in:Ref.6
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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