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VLDLR elisa kit :: Mouse Very low density lipoprotein receptor ELISA Kit

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Catalog # MBS721361
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Very low density lipoprotein receptor (VLDLR), ELISA Kit

Popular Item
Full Product Name

Mouse Very low density lipoprotein receptor ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
gene 224050
Species Reactivity
This assay has high sensitivity and excellent specificity for detection of VLDLR. No significant cross-reactivity or interference between VLDLR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between VLDLR and all the analogues, therefore, cross reaction may still exist in some cases.
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Detection Range
Intended Uses
This VLDLR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse VLDLR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of VLDLR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for VLDLRpurchase
MBS721361 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Very low density lipoprotein receptor (VLDLR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing VLDLR. The ELISA analytical biochemical technique of the MBS721361 kit is based on VLDLR antibody-VLDLR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VLDLR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VLDLR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
VLDLR elisa kit
Principle of the assay: VLDLR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-VLDLR antibody and an VLDLR-HRP conjugate. The assay sample and buffer are incubated together with VLDLR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VLDLR concentration since VLDLR from samples and VLDLR-HRP conjugate compete for the anti-VLDLR antibody binding site. Since the number of sites is limited, as more sites are occupied by VLDLR from the sample, fewer sites are left to bind VLDLR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VLDLR concentration in each sample is interpolated from this standard curve.
Product Categories/Family for VLDLR elisa kit

Typical Testing Data/Standard Curve (for reference only) of VLDLR elisa kit
VLDLR elisa kit Typical Testing Data/Standard Curve (for reference only) image
NCBI/Uniprot data below describe general gene information for VLDLR. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
93,383 Da
NCBI Official Full Name
very low-density lipoprotein receptor isoform a
NCBI Official Synonym Full Names
very low density lipoprotein receptor
NCBI Official Symbol
VLDLR  [Similar Products]
NCBI Official Synonym Symbols
  [Similar Products]
NCBI Protein Information
very low-density lipoprotein receptor; VLDL-R; VLDL receptor
UniProt Protein Name
Very low-density lipoprotein receptor
UniProt Gene Name
VLDLR  [Similar Products]
UniProt Synonym Gene Names
VLDL receptor; VLDL-R  [Similar Products]
UniProt Entry Name
NCBI Summary for VLDLR
The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. This gene encodes a lipoprotein receptor that is a member of the LDLR family and plays important roles in VLDL-triglyceride metabolism and the reelin signaling pathway. Mutations in this gene cause VLDLR-associated cerebellar hypoplasia. Alternative splicing generates multiple transcript variants encoding distinct isoforms for this gene. [provided by RefSeq, Aug 2009]
UniProt Comments for VLDLR
VLDLR: Binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation. Defects in VLDLR are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 1 (CMARQ1); also known as dysequilibrium syndrome (DES) or non- progressive cerebellar disorder with mental retardation. CMARQ1 is a congenital, non-progressive cerebellar ataxia associated with disturbed equilibrium, delayed ambulation, mental retardation and cerebellar hypoplasia. Additional features include short stature, strabismus, pes planus and, rarely, seizures. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p24

Cellular Component: membrane; integral to membrane; plasma membrane; coated pit; receptor complex

Molecular Function: very-low-density lipoprotein receptor activity; low-density lipoprotein receptor activity; very-low-density lipoprotein binding; protein binding; apolipoprotein binding; calcium ion binding; glycoprotein binding; calcium-dependent protein binding

Biological Process: receptor-mediated endocytosis; nervous system development; cholesterol metabolic process; positive regulation of protein kinase activity; ventral spinal cord development; negative regulation of transcription from RNA polymerase II promoter; lipid transport; signal transduction; memory

Disease: Cerebellar Ataxia, Mental Retardation, And Dysequilibrium Syndrome 1
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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