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VTN elisa kit :: vitronectin total antigen assay ELISA Kit

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Catalog # MBS135526
Unit / Price
  1 Kit  /  $540 +1 FREE 8GB USB
  5 Kits  /  $1,980 +2 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

vitronectin total antigen assay (VTN), ELISA Kit

Also Known As

Mouse vitronectin total antigen assay ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
478
Chromosome Location
Chromosome: 11; NC_000077.6 (78499120..78502325). Location: 11 B5; 11 46.74 cM
3D Structure
ModBase 3D Structure for P29788
Host
Multiple
Form/Format
Complete Kit
Samples
Biological fluids
Intra-assay Precision
These studies are currently in progress. Please contact us for more information.
Inter-assay Precision
These studies are currently in progress. Please contact us for more information.
Intended Uses
This mouse vitronectin total antigen assay is for the quantitative determination of total vitronectin antigen in biological fluids. For research use only.
Preparation and Storage
Store at 4 degree C. Shelf Life: 6 months from manufacture, see label for expiration date
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of VTN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forVTNpurchase
MBS135526 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Multiple vitronectin total antigen assay (VTN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing VTN. The ELISA analytical biochemical technique of the MBS135526 kit is based on VTN antibody-VTN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VTN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VTN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
VTN elisa kit
Principle of the Assay: Mouse vitronectin will bind to the affinity purified capture antibody coated on the microtiter plate. Free and ligand bound enzyme will react with the antibody on the plate. After appropriate washing steps, biotinylated anti-mouse vitronectin primary antibody binds to the captured protein. Excess antibody is washed away and bound polyclonal antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse vitronectin. The amount of color development is directly proportional to the concentration of total vitronectin in the sample.
Background: Vitronectin is an abundant plasma glycoprotein that helps regulate coagulation, fibrinolysis, complement activation, and cell adhesion [1,3,7]. Vitronectin binds to glycosaminoglycans, collagen, plasminogen and urokinase receptors. It also may control the clearance of vascular thrombi by binding and stabilizing PAI-1. In binding PAI-1, it extends the lifetime of active PAI-1 [4,5]. Vitronectin may also be involved in the regulation of bone metabolism [2].

Typical Testing Data/Standard Curve (for reference only) of VTN elisa kit
VTN elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS135526. Click to request current manual
NCBI/Uniprot data below describe general gene information for VTN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
54,849 Da
NCBI Official Full Name
vitronectin
NCBI Official Synonym Full Names
vitronectin
NCBI Official Symbol
NCBI Official Synonym Symbols
Vn; AI256434
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NCBI Protein Information
vitronectin; S-protein; serum-spreading factor
UniProt Protein Name
Vitronectin
UniProt Synonym Protein Names
S-protein; Serum-spreading factor
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
VTNC_MOUSE
UniProt Comments for VTN
VTN: Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Cell adhesion; Secreted

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: integrin binding; heparin binding; extracellular matrix binding; scavenger receptor activity; polysaccharide binding

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; extracellular matrix organization and biogenesis; positive regulation of protein binding; cell-matrix adhesion; cell adhesion mediated by integrin; immune response; positive regulation of smooth muscle cell migration; cell adhesion; positive regulation of receptor-mediated endocytosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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