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VZV ORF9 recombinant protein :: Varicella Zoster Virus ORF9 Recombinant Protein

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Catalog # MBS553166
Unit / Price
  0.5 mg  /  $775 +1 FREE 8GB USB
  1 mg  /  $1,235 +1 FREE 8GB USB
VZV ORF9 recombinant protein
Product Name

Varicella Zoster Virus ORF9 (VZV ORF9), Recombinant Protein

Also Known As

Varicella Zoster Virus ORF9 Recombinant

Product Synonym Names
vzv; orf9; varicella zoster
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
302
3D Structure
ModBase 3D Structure for Q4JQW6
Host
E Coli
Purity/Purification
>95%, as determined by SDS-PAGE.
Form/Format
Lyophilized from 25mM Tris-Hcl pH 8.0, 1mM EDTA and 50% glycerol.
Host Note
DNA sequence encoding immunodominant fragment of Varicella Zoster virus ORF9 protein was expressed in Escherichia Coli.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Recombinant Varicella Zoster virus ORF9 encodes the immunodominant sequences of the VZV ORF9 protein. This molecule has a calculated mass of approximately 5kDa.

Recombinant VZV ORF9 contains an N terminal GST purification tag and migrates as an approximately 31 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this protein can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of VZV ORF9 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
VZV ORF9 recombinant protein
The varicella-zoster virus (VZV) ORF9 protein is a member of the herpesvirus UL49 gene family but shares limited identity and similarity with the UL49 prototype, herpes simplex virus type 1 VP22. ORF9 mRNA is the most abundantly expressed message during VZV infection. The VZV ORF9 protein has not been well characterized, despite the fact that its transcript is the most abundant viral message expressed during lytic infection.
Product Categories/Family for VZV ORF9 recombinant protein
Application Notes for VZV ORF9 recombinant protein
VZV ORF9 antigen is suitable for ELISA and Western blots, excellent antigen for detection of VZV with minimal specificity problems.
NCBI/Uniprot data below describe general gene information for VZV ORF9. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
UniProt Primary Accession #
Molecular Weight
32,816 Da
NCBI Official Full Name
Tegument protein VP22
UniProt Protein Name
Tegument protein VP22
UniProt Synonym Protein Names
ORF9 protein; Tegument protein 9
UniProt Entry Name
VP22_VZVO
UniProt Comments for VZV ORF9
Function: Tegument protein that may play a role in the nucleocapsid secondary envelopment at the trans-Golgi network during virus morphogenesis

Potential.

Subunit structure: Interacts with gE (via C-terminus); this interaction is necessary for the recruitment of VP22 to the Golgi and its packaging into virions. Interacts with VP16

By similarity. Ref.3

Subcellular location: Virion tegument

By similarity. Host cytoplasm

By similarity. Host nucleus

By similarity. Host Golgi apparatus

By similarity. Note: One of the most abundant tegument protein. During virion morphogenesis, this protein probably accumulates at the trans-Golgi where secondary envelopment occurs

By similarity. Ref.3

Post-translational modification: Highly phosphorylated in the host cell. Packaging is selective for underphosphorylated forms

By similarity.

Sequence similarities: Belongs to the alphaherpesvirinae VP22 tegument protein family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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