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WASF3 elisa kit :: Human WAS protein family, member 3 ELISA Kit

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Catalog # MBS9316818
Unit / Price
  48-Strip-Wells  /  $470 +1 FREE 8GB USB
  96-Strip-Wells  /  $680 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
WASF3 elisa kit
Product Name

WAS protein family, member 3 (WASF3), ELISA Kit

Popular Item
Full Product Name

Human Wiskott-Aldrich syndrome protein family member 3, WASF3 ELISA Kit

Product Synonym Names
Human Wiskott-Aldrich syndrome protein family member 3 (WASF3) ELISA kit; RP11-496O3.2; Brush-1; KIAA0900; SCAR3; WAVE3; WASP family Verprolin-homologous protein 3; WAS protein family; member 3
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
605068
3D Structure
ModBase 3D Structure for Q9UPY6
Species Reactivity
Specificity
No significant cross-reactivity or interference between Human WASF3 and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces and Urine
Assay Type
Sandwich
Detection Range
31.2 pg/ml - 1000 pg/ml.
Sensitivity
5.0 pg/ml.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated WASF3 concentrations in Human serum, plasma and other body fluids. Using Purified Human WASF3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add WASF3 and WASF3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of WASF3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of WASF3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forWASF3purchase
MBS9316818 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the WAS protein family, member 3 (WASF3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WASF3. The ELISA analytical biochemical technique of the MBS9316818 kit is based on WASF3 antibody-WASF3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WASF3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WASF3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
WASF3 elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated WASF3 concentrations in Human serum, plasma and other body fluids. Using Purified Human WASF3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add WASF3 and WASF3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of WASF3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS9316818. Click to request current manual
NCBI/Uniprot data below describe general gene information for WASF3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
55,293 Da
NCBI Official Full Name
wiskott-Aldrich syndrome protein family member 3 isoform 1
NCBI Official Synonym Full Names
WAS protein family, member 3
NCBI Official Symbol
WASF3  [Similar Products]
NCBI Official Synonym Symbols
SCAR3; WAVE3; Brush-1
  [Similar Products]
NCBI Protein Information
wiskott-Aldrich syndrome protein family member 3; protein WAVE-3; WASP family Verprolin-homologous protein 3; verprolin homology domain-containing protein 3
UniProt Protein Name
Wiskott-Aldrich syndrome protein family member 3
UniProt Synonym Protein Names
Protein WAVE-3; Verprolin homology domain-containing protein 3
UniProt Gene Name
WASF3  [Similar Products]
UniProt Synonym Gene Names
KIAA0900; SCAR3; WAVE3; WASP family protein member 3  [Similar Products]
UniProt Entry Name
WASF3_HUMAN
NCBI Summary for WASF3
This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. A pseudogene of this gene have been defined on chromosome 6. Alternative splicing results in multiple transcript variants [provided by RefSeq, May 2014]
UniProt Comments for WASF3
WASF3: Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. Binds actin and the Arp2/3 complex. Expressed in ovary and brain. Belongs to the SCAR/WAVE family.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: cytoskeleton; lamellipodium; cytoplasm

Molecular Function: actin binding

Biological Process: positive regulation of myelination; lamellipodium biogenesis; regulation of cell shape; actin filament polymerization; cytoskeleton organization and biogenesis; protein complex assembly; oligodendrocyte development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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