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WASL peptide :: WASP / N-WASP Peptide

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Catalog # MBS474069
Unit / Price
  0.05 mg  /  $130 +1 FREE 8GB USB
WASL peptide
Product Name

WASP / N-WASP Peptide (WASL), Peptide

Also Known As

WASP / N-WASP Blocking Peptide

Product Synonym Names
WASP / N-WASP Peptide
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
505
Sequence
Peptide Sequence: WASP / N-WASP synthetic peptide correspond s to a region near the C-terminus of human WASP. This sequence is highly homologous with a similar region in human N-WASP.
Chromosome Location
Chromosome: 7; NC_000007.13 (123321997..123389116, complement). Location: 7q31.3
OMIM
605056
3D Structure
ModBase 3D Structure for O00401
Specificity
The peptide is specifically recognized by anti-WASP/N-WASP antibody (WP2101) in ELISA, and has been shown to block the reactivity of WP2101 during Western blot. In addition, the peptide is recommended for use in blocking WP2101 reactivity in immunocytochemistry.
Form/Format
Blocking Peptide is supplied in 50ul phosphate-buffered saline and 0.05% sodium azide.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Other Notes
Small volumes of WASL peptide vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
WASL peptide
Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Serine and tyrosine phosphorylation regulates the activity of both proteins. WASP is observed as a 63 kDa protein in hematopoietic cells, while N-WASP is observed as a 65 kDa in many tissues, especially brain.
Application Notes for WASL peptide
Antibody Blocking: 1 ug/ml
ELISA: 10-100 ng/well
NCBI/Uniprot data below describe general gene information for WASL. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
NCBI Official Full Name
neural Wiskott-Aldrich syndrome protein
NCBI Official Synonym Full Names
Wiskott-Aldrich syndrome-like
NCBI Official Symbol
NCBI Official Synonym Symbols
NWASP; N-WASP; MGC48327; DKFZp779G0847
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NCBI Protein Information
neural Wiskott-Aldrich syndrome protein; OTTHUMP00000211994; Wiskott-Aldrich syndrome gene-like
UniProt Protein Name
Neural Wiskott-Aldrich syndrome protein
UniProt Gene Name
UniProt Entry Name
WASL_HUMAN
NCBI Summary for WASL
The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes. [provided by RefSeq]
UniProt Comments for WASL
Function: Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. Ref.11

Subunit structure: Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with SNX9. Interacts with the WW domains of PRPF40A/FBP11

By similarity. Interacts with NOSTRIN. Interacts with Shigella flexneri protein icsA. The interaction with icsA enhances the affinity of WASL for Arp2/3, thus assembling a tight complex which has maximal activity in actin assembly. Interacts with E.coli EspF-like protein encoded on prophage U. Identified in a complex containing at least WASL, BAIAP2L1 and E.coli EspF-like protein encoded on prophage U. Ref.4 Ref.5 Ref.6 Ref.11

Subcellular location: Cytoplasm › cytoskeleton

By similarity. Nucleus

By similarity. Note: Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated

Sequence similarities: Contains 1 CRIB domain.Contains 1 WH1 domain.Contains 2 WH2 domains.
Product References and Citations for WASL peptide
• Cory, G.O. et al. (2003) Mol Cell. 11(5):1229-39.
• Higgs, H.N. & Pollard, T.D. (2001) Annu Rev Biochem 70:649-676.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Products associated withWASL peptide
Organs/Tissues associated with WASL peptide
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