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WIF1 elisa kit :: Mouse Wnt inhibitory factor 1 ELISA Kit

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Catalog # MBS282004
Unit / Price
  48-Strip-Wells  /  $405 +1 FREE 8GB USB
  96-Strip-Wells  /  $675 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,080 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,805 +7 FREE 8GB USB
WIF1 elisa kit
Product Name

Wnt inhibitory factor 1 (WIF1), ELISA Kit

Popular Item
Full Product Name

Mouse Wnt inhibitory factor 1 (WIF1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for Q9WUA1
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse WIF1. No significant cross-reactivity or interference between Mouse WIF1 and analogues was observed.
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of WIF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forWIF1purchase
MBS282004 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Wnt inhibitory factor 1 (WIF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WIF1. The ELISA analytical biochemical technique of the MBS282004 kit is based on WIF1 antibody-WIF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WIF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WIF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
WIF1 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate WIF1 in samples. An antibody specific for WIF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWIF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WIF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WIF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS282004. Click to request current manual
NCBI/Uniprot data below describe general gene information for WIF1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
41,590 Da
NCBI Official Full Name
wnt inhibitory factor 1
NCBI Official Synonym Full Names
Wnt inhibitory factor 1
NCBI Official Symbol
NCBI Official Synonym Symbols
WIF-1; AW107799
  [Similar Products]
NCBI Protein Information
wnt inhibitory factor 1
UniProt Protein Name
Wnt inhibitory factor 1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
WIF-1  [Similar Products]
UniProt Comments for WIF1
WIF1: Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10|10 D2

Cellular Component: extracellular region

Molecular Function: Wnt-protein binding

Biological Process: multicellular organism development; negative regulation of Wnt signaling pathway; positive regulation of fat cell differentiation; Wnt signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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