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WNT11 elisa kit :: Human Protein Wnt-11 ELISA Kit

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Catalog # MBS281148
Unit / Price
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  48-Strip-Wells  /  $405 +1 FREE 8GB USB
  96-Strip-Wells  /  $675 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,080 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,805 +7 FREE 8GB USB
WNT11 elisa kit
Product Name

Protein Wnt-11 (WNT11), ELISA Kit

Popular Item
Full Product Name

Human Protein Wnt-11 (WNT11) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
3D Structure
ModBase 3D Structure for O96014
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human WNT11. No significant cross-reactivity or interference between Human WNT11 and analogues was observed.
Samples
serum, plasma, cell culture supernates and other biological fluids
Detection Range
31.25 pg/mL - 2000 pg/mL
Sensitivity
15.6 pg/mL (mean of 6 independent assays).
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of WNT11 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for WNT11purchase
MBS281148 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Protein Wnt-11 (WNT11) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WNT11. The ELISA analytical biochemical technique of the MBS281148 kit is based on WNT11 antibody-WNT11 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WNT11 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WNT11. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
WNT11 elisa kit
Intended Uses: For the quantitative detection of Human Protein Wnt-11 (WNT11) concentration in serum, plasma, cell culture supernates and other biological fluids.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate WNT11 in Human serum, plasma, cell culture supernates. An antibody specific for WNT11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT11 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS281148. Click to request current manual
NCBI/Uniprot data below describe general gene information for WNT11. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
39,179 Da
NCBI Official Full Name
protein Wnt-11
NCBI Official Synonym Full Names
Wnt family member 11
NCBI Official Symbol
WNT11  [Similar Products]
NCBI Official Synonym Symbols
HWNT11
  [Similar Products]
NCBI Protein Information
protein Wnt-11
UniProt Protein Name
Protein Wnt-11
Protein Family
UniProt Gene Name
WNT11  [Similar Products]
NCBI Summary for WNT11
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 97%, 85%, and 63% amino acid identity with mouse, chicken, and Xenopus Wnt11 protein, respectively. This gene may play roles in the development of skeleton, kidney and lung, and is considered to be a plausible candidate gene for High Bone Mass Syndrome. [provided by RefSeq, Jul 2008]
UniProt Comments for WNT11
WNT11: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q13.5

Cellular Component: cytoplasm; extracellular region; extracellular space; proteinaceous extracellular matrix

Molecular Function: frizzled binding; GTPase activator activity; protein binding; protein kinase activator activity

Biological Process: activation of protein kinase activity; adrenal gland development; artery morphogenesis; bone mineralization; cell fate commitment; cloacal septation; convergent extension involved in axis elongation; embryonic skeletal system development; epithelial to mesenchymal transition; maintenance of epithelial cell polarity; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of cell migration; negative regulation of transcription, DNA-dependent; neuron differentiation; notochord morphogenesis; osteoblast differentiation; palate development; paraxial mesoderm formation; positive regulation of apoptosis; positive regulation of cell migration; positive regulation of GTPase activity; positive regulation of stress fiber formation; positive regulation of transcription, DNA-templated; positive regulation of transforming growth factor beta2 production; protein amino acid phosphorylation; response to nutrient levels; Wnt receptor signaling pathway through beta-catenin; Wnt receptor signaling pathway, calcium modulating pathway; Wnt receptor signaling pathway, planar cell polarity pathway; Wnt signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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